Author(s): Machado, Jorge ; Gato, Inês ; Lopes, Teresa ; Pereira, Teresa ; Santos, Mário ; Oleastro, Mónica ; Chaves, Paula ; Guerreiro, António
Date: 2011
Persistent ID: http://hdl.handle.net/10400.18/362
Origin: Repositório Científico do Instituto Nacional de Saúde
Subject(s): Helicobacter pylori; Intestinal microbiota; Infecções Gastrointestinais
Intestinal microbiota may influence inflammation in the host. The aim of the present study was to explore the role of modulation of intestinal microbiota in the outcome in the Helicobacter pylori (Hp) gastric inflammation. Twenty five C57BL/6 male mice were separated in three groups: Control group (CG) n = 5 Infected group (IG) n = 10 and Synbiotic 2000TM (SG) n = 10. CG received PBS by gavage; IG and SG were inoculated intragastrically with H. pylori SS1 cell suspension (109 CFU/mL). Then, mice were treated either with PBS (CG and IG) or Synbiotic 2000TM (SG). Five mice from each group were sacrificed at week 6 and the other at week 18. At each time samples were collected from: gastric tissue to immunohistochemistry and histological evaluation (HE) and faeces to evaluate intestinal microbiota composition by FISH, targeting 14 bacterial groups. IG and SG groups were H. pylori positive by immunohistochemistry. Microbiota analysis: In IG there were significant changes in the microbiota composition, comparing to CG. At week 6 there were changes in 12 of 14 (85.7%) bacterial groups, while at week 18 there was a change in 6/14 (42.9%). In SG, there were changes in 7/14 (50.0%) at week 6, and in 4/14 (28.6%) at week 18, comparing to CG. Histology: IG at weeks 6 and 18 has 40% (2/5) of intramucosal inflammation and SG at the same end points has 0% (0/5). These results suggest that modulation of the intestinal microbiota by Synbiotic 2000TM may influence the outcome of Hp gastric inflammation.
