Author(s):
Loureiro, Ana Isabel Sá ; Nogueira, Eugénia Sofia Costa ; Azóia, Nuno G. ; Sárria, M. P. ; Abreu, Ana S. ; Shimanovich, Ulyana ; Rollett, Alexandra ; Härmark, Johan ; Hebert, Hans ; Guebitz, Georg ; Bernardes, Gonçalo J. L. ; Preto, Ana ; Gomes, Andreia C. ; Cavaco-Paulo, Artur
Date: 2015
Persistent ID: http://hdl.handle.net/1822/37157
Origin: RepositóriUM - Universidade do Minho
Subject(s): Protein-based nanoemulsions; High pressure homogenization; PEGylated surfactant; Folic acid; Active targeting; Science & Technology; Ciências Médicas::Biotecnologia Médica
Description
Bovine Serum Albumin (BSA) nanoemulsions were produced by high pressure homogenization with a tri-block copolymer (Poloxamer 407), which presents a central hydrophobic chain of polyoxypropylene (PPO) and two identical lateral hydrophilic chains of polyethylene glycol (PEG). We observed a linear correlation between tri-block copolymer concentration and size - the use of 5 mg/mL of Poloxamer 407 yields nanoemulsions smaller than 100 nm. Molecular dynamics and fluorescent tagging of the tri-block copolymer highlight their mechanistic role on the size of emulsions. This novel method enables the fabrication of highly stable albumin emulsions in the nano-size range, highly desirable for controlled drug delivery. Folic Acid (FA)-tagged protein nanoemulsions were shown to promote specific Folate Receptor (FR)-mediated targeting in FR positive cells. The novel strategy presented here enables the construction of size controlled, functionalized protein-based nanoemulsions with excellent characteristics for active targeting in cancer therapy.
European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement NMP4-LA-2009-228827 NANOFOL. This work was supported by FEDER through POFC – COMPETE and by Portuguese funds from FCT through the project PEst-OE/BIA/UI4050/2014. The authors also thank the FCT Strategic Project of UID/BIO/04469/2013 unit.
