Author(s):
Santos, Tiago dos ; Tavares, Catarina ; Sousa, Diana ; Vaz, Josiana A. ; Calhelha, Ricardo C. ; Martins, Anabela ; Almeida, Gabriela M. ; Ferreira, Isabel C. F. R. ; Vasconcelos, M. Helena
Date: 2013
Persistent ID: http://hdl.handle.net/1822/63961
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/110062/PT
;
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F43653%2F2008/PT;
Subject(s): Suillus luteus; Methanolic extract; Tumor cell growth; Tumor cell proliferation; Science & Technology
Description
Several edible mushrooms extracts are known to have tumor cell growth inhibitory potential. The objective of this work was to study this potential in extracts of Suillus luteus collected from the Northeast of Portugal. Various extracts were prepared and their effect on tumor cell growth was studied with the SRB assay in four human tumor cell lines: MCF-7 (breast), NCI-H460 (non-small cell lung cancer), AGS (gastric) and HCT-15 (colon). The methanolic extract of S. luteus was the most potent extract. This extract was slightly more potent in the HCT-15 cells (with mutant p53, GI(50) = 17.8 +/- 1.6 mu g/mL) than in the other cell lines tested, which suggested that its effect was not p53-dependent. Indeed, in HCT-15 cells, an increase in the levels of p53 was detected but no alterations in some of the proteins transactivated by p53 (e.g. p21 or Box) were found. The extract caused an increase in the cellular levels of p-H2A.X, which is suggestive of DNA damage. Growth inhibition in these cells was mostly due to inhibition of cell proliferation and an increase in the % of cell in the G1 phase of the cell cycle. An increase in cell death was also found, even though to very low levels. In addition, this extract was not cytotoxic to primary cultures of porcine hepatocytes (GI(50) > 400 mu g/mL). Together, these results indicate that the S. luteus methanolic extract may be an interesting source of compounds that inhibit the proliferation of tumor cells but further studies should be carried out in order to understand its potential.
The authors are grateful to Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) and COMPETE/QREN/EU for the financial support to this work (research project PTDC/AGR-ALI/110062/2009) and to CIMO (strategic project PEst-OE/AGR/U10690/2011). They also thank the University of Porto and Santander Totta for financial support. J.A. Vaz thanks FCT, POPH-QREN and FSE for her grant (BD/43653/2008). G.M. Almeida is supported by FCT and the European Social Fund. IPATIMUP is an Associate Laboratory of the Portuguese Ministry of Science, Technology and Higher Education and is partially supported by FCT.
