Author(s):
Teixeira, Marta Albertina ; Antunes, Joana Isabel Costa ; Seabra, Catarina Leal ; Tohidi, Shafagh Dinparast ; Reis, Salette ; Amorim, M. T. Pessoa de ; Felgueiras, Helena Prado
Date: 2022
Persistent ID: https://hdl.handle.net/1822/77786
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCTM-TEX%2F28074%2F2017/PT;
info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FNAN-MAT%2F31444%2F2017/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00264%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT;
info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F148930%2F2019/PT;
Subject(s): Accelerated clotting; Adjuvant action; Antimicrobial peptides; Electrospun nanofibers; Enhanced antibacterial effect; Surface immobilization; Science & Technology; Saúde de qualidade; Engenharia e Tecnologia::Engenharia dos Materiais
Description
In this research, we propose to engineer a nanostructured mat that can simultaneously kill bacteria and promote an environment conducive to healing for prospective wound care. Polyvinyl alcohol (PVA) and cellulose acetate (CA) were combined at different polymer ratios (100/0, 90/10, 80/20% v/v), electrospun and crosslinked with glutaraldehyde vapor. Crosslinked fibers increased in diameter (from 194 to 278 nm), retaining their uniform structure. Fourier-transform infrared spectroscopy and thermal analyses proved the excellent miscibility between polymers. CA incorporation incremented the fibers swelling capacity and reduced the water vapor and air permeabilities of the mats, preventing the excessive drying of wounds. The antimicrobial peptide cys-pexiganan and the immunoregulatory peptide Tiger 17 were incorporated onto the mats via polyethylene glycol spacer (hydroxyl-PEG2-maleimide) and physisorbed, respectively. Time-kill kinetics evaluations revealed the mats effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa. Tiger 17 played a major role in accelerating clotting of re-calcified plasma. Data reports for the first time the collaborative effect of pexiganan and Tiger 17 against bacterial infections and in boosting hemostasis. Cytocompatibility data verified the peptide-modified mats safety. Croslinked 90/10 PVA/CA mats were deemed the most promising combination due to their moderate hydrophilicity and permeabilities, swelling capacity, and high yields of peptide loading.
This research received funding from the Portuguese Foundation for Science and Technology (FCT) under the scope of the projects PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074), PTDC/NANMAT/31444/2017 (POCI-01-0145-FEDER-031444), UID/CTM/00264/2020, UIDB/50006/2020 and SFRH/BD/148930/2019.
Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the projects with reference PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074) and PTDC/NANMAT/31444/2017 (POCI-01-0145-FEDER-031444). Authors also acknowledge project UID/CTM/00264/2020 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTES. M.A.T. acknowledges FCT for the PhD grant with reference SFRH/BD/148930/2019. The authors acknowledge to the institution LAQV – Laboratório Associado para a Química Verde (UIDB/50006/2020).
