Author(s):
Singhal, Jaron ; Pinho, Diana ; Lopes, Raquel ; Sousa, Patrícia C. ; Garcia, Valdemar ; Schütte, Helmut ; Lima, Rui A. ; Gassmann, Stefan
Date: 2015
Persistent ID: http://hdl.handle.net/10198/16154
Origin: Biblioteca Digital da UPB
Project/scholarship:
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC/SAU-ENB/116929/2010/PT;
info:eu-repo/grantAgreement/FCT/COMPETE/EXPL/EMS-SIS/2215/2013/PT;
info:eu-repo/grantAgreement/FCT/SFRH/SFRH/BD/89077/2012/PT;
info:eu-repo/grantAgreement/FCT//SFRH/BPD/75258/2010/PT;
Subject(s): Biomedical applications; Blood cells; Cell-free layer; Cells separation; Microfluidic devices; Micromilling
Description
The most common and used technique to produce microfluidic devices for biomedical applications is the soft-lithography. However, this is a high cost and time-consuming technique. Recently, manufacturers were able to produce milling tools smaller than 100 μm and consequently have promoted the ability of the micromilling machines to fabricate microfluidic devices capable of performing cell separation. In this work, we show the ability of a micromilling machine to manufacture microchannels down to 30 μm and also the ability of a microfluidic device to perform partial separation of red blood cells from plasma. Flow visualization and measurements were performed by using a high-speed video microscopy system. Advantages and limitations of the micromilling fabrication process are also presented.
