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Internal Validation of the Investigator 26Plex QS Amplification Kit: a high-throughput multiplex assay for reference and low copy number DNA samples

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Detalhes bibliográficos
Resumo:Human singularity provides a foundation for forensic genetics, where the study of Short Tandem Repeats (STRs) is key. Furthermore, the development of polymerase chain reaction (PCR) has revolutionized the field by enabling the amplification of specific DNA markers. The Investigator® 26plex QS Amplification Kit is a highly robust multiplex PCR system, for human identification. In this study, we report the results obtained from the internal validation carried out at the Portuguese National Institute of Legal Medicine and Forensic Sciences. The results showed that the Investigator® 26plex QS kit proved to be a robust tool for the analysis of forensic samples, offering high sensitivity and specificity.
Autores principais:Neves Cardoso, Paula Liliana
Outros Autores:Serra, Armando; Bogas, Vanessa; Balsa, Filipa; Lopes, Virginia; Dario, Rita; Porto, Maria João; Amorim, António; Corte Real, Francisco; Brito, Pedro
Assunto:Validation Amplification Kit Thresholds STRs
Ano:2025
País:Portugal
Tipo de documento:artigo original
Tipo de acesso:acesso restrito
Instituição associada:Instituto Nacional de Medicina Legal e Ciências Forenses, IP
Idioma:inglês
Origem:Instituto Nacional de Medicina Legal e Ciências Forenses, IP
Descrição
Resumo:Human singularity provides a foundation for forensic genetics, where the study of Short Tandem Repeats (STRs) is key. Furthermore, the development of polymerase chain reaction (PCR) has revolutionized the field by enabling the amplification of specific DNA markers. The Investigator® 26plex QS Amplification Kit is a highly robust multiplex PCR system, for human identification. In this study, we report the results obtained from the internal validation carried out at the Portuguese National Institute of Legal Medicine and Forensic Sciences. The results showed that the Investigator® 26plex QS kit proved to be a robust tool for the analysis of forensic samples, offering high sensitivity and specificity.