Publicação
Pseudofistulina radicata (Schwein) Burds: the first study focused on its antioxidante activity and bioactive compounds
| Resumo: | Natural products with antioxidant activity are very useful in helping the organism to maintain health and be protected from several diseases originated by the excessive production of free radicais, being also used as nutraceuticals. Accordingly, the antioxidants intake from our daily diet, particularly in mushrooms, is of great importance, acting as protective agents against oxidative damage [1]. Pseudofistulina radicata (Schwein) Burds is a wild native mushroom from El Salvador, of which to the best of our knowledge no studies have been published identifying bioactive molecules or evaluating its antioxidant properties. The present work aims to study the antioxidant activity and to identify the bioactive compounds present in the dichloromethane extract obtained from this mushroom. P. radicata was purchase from street vendors in the municipality of Concepción de Ataco (Ahuachapán, El Salvador). An increasing polarity extraction from powdered dry mushroom, with tive solvents at room temperature from hexane to water, was performed. The antioxidant activity of the dichloromethane extract (the second one after using hexane) was evaluated through the reducing power and p-carotene bleaching inhibition assays; the results were 1.43 ± 0.01mg/mL responsible for 0.5 absorbance in the reducing power assay and 2.50 ± O.OSmg/mL responsible for 50% of pcarotene bleaching inhibition. The fraction was chemically characterized by gás chromatography coupled to a mass spectrometer detector (GC-MS) and proton nuclear magnetic resonance ('H NMR). Palmitic acid, linoleic acid and friedelin were the main molecules identified. Linoleic acid is an essential fatty acid, presenting anti-inflammatory activity and that prevents coronary diseases [2]. Friedelin is a terpene which possesses analgesic, anti-inflammatory and antipyretic activities [3]. Overall, these results indicate and strong potential of P. radicata to be used as a source of bioactive molecules, which could be included in diets as nutraceuticals and/or as functional foods maintaining and promoting health, longevity and life quality. |
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| Autores principais: | Castaneda Najarro, Blanca Cecilia |
| Outros Autores: | Fernandes, Ângela; Barros, Lillian; Heleno, Sandrina A.; García, Pablo A.; Castro, María Ángeles; Ferreira, Isabel C.F.R. |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | Natural products with antioxidant activity are very useful in helping the organism to maintain health and be protected from several diseases originated by the excessive production of free radicais, being also used as nutraceuticals. Accordingly, the antioxidants intake from our daily diet, particularly in mushrooms, is of great importance, acting as protective agents against oxidative damage [1]. Pseudofistulina radicata (Schwein) Burds is a wild native mushroom from El Salvador, of which to the best of our knowledge no studies have been published identifying bioactive molecules or evaluating its antioxidant properties. The present work aims to study the antioxidant activity and to identify the bioactive compounds present in the dichloromethane extract obtained from this mushroom. P. radicata was purchase from street vendors in the municipality of Concepción de Ataco (Ahuachapán, El Salvador). An increasing polarity extraction from powdered dry mushroom, with tive solvents at room temperature from hexane to water, was performed. The antioxidant activity of the dichloromethane extract (the second one after using hexane) was evaluated through the reducing power and p-carotene bleaching inhibition assays; the results were 1.43 ± 0.01mg/mL responsible for 0.5 absorbance in the reducing power assay and 2.50 ± O.OSmg/mL responsible for 50% of pcarotene bleaching inhibition. The fraction was chemically characterized by gás chromatography coupled to a mass spectrometer detector (GC-MS) and proton nuclear magnetic resonance ('H NMR). Palmitic acid, linoleic acid and friedelin were the main molecules identified. Linoleic acid is an essential fatty acid, presenting anti-inflammatory activity and that prevents coronary diseases [2]. Friedelin is a terpene which possesses analgesic, anti-inflammatory and antipyretic activities [3]. Overall, these results indicate and strong potential of P. radicata to be used as a source of bioactive molecules, which could be included in diets as nutraceuticals and/or as functional foods maintaining and promoting health, longevity and life quality. |
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