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Phenylethanoid glycosides in Veronica urticifolia extracts: characterization by HPLC-DAD-ESI/MS

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Detalhes bibliográficos
Resumo:Veronica (Piantaginaceae) is a genus of 450 species found in temperate regions of both hemispheres. ln this work, the aerial parts of Veronica urticifolia were collected during flowering period from Mountain Goc in central Serbia. Powdered samples (1 g) were extracted twice with methanol:water 80:20 (v/v). After removing solvents, the combined extracts were re-dissolved in 20% aqueous methanol, filtered through 0.22-1-lm disposable LC filter disks and analyzed by high performance liquid chromatography coupled to a diode array detector and electrospray-mass spectrometry (HPLC-DAD/ESI-MS). The phenolic compounds were characterized according to their UV and mass spectra and retention times. For the quantitative analysis, calibration curves of standard compounds were used.
Autores principais:Barreira, João C.M.
Outros Autores:Stojković, Dejan; Maksimović, Zoran; Živković, Jelena; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Ano:2013
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
Descrição
Resumo:Veronica (Piantaginaceae) is a genus of 450 species found in temperate regions of both hemispheres. ln this work, the aerial parts of Veronica urticifolia were collected during flowering period from Mountain Goc in central Serbia. Powdered samples (1 g) were extracted twice with methanol:water 80:20 (v/v). After removing solvents, the combined extracts were re-dissolved in 20% aqueous methanol, filtered through 0.22-1-lm disposable LC filter disks and analyzed by high performance liquid chromatography coupled to a diode array detector and electrospray-mass spectrometry (HPLC-DAD/ESI-MS). The phenolic compounds were characterized according to their UV and mass spectra and retention times. For the quantitative analysis, calibration curves of standard compounds were used.