Publicação
Chemical characterization and bioactivity of poplar, green and red propolis: a screening study with a food preservation purpose
| Resumo: | Propolis is a resin made by honeybees from multiple plant sources surrounding the hive. Therefore, each type of propolis from different regions has intrinsic characteristics related to their chemical composition, such as antifungal, antioxidative properties, and extrinsic such as color, both specific to their botanical origin. Thus, propolis can be regarded as a functional food with preservative properties offering an alternative to the concern of the global and growing use of synthetic preservatives considerably harming human health. The aim of this work was to study three types of propolis, namely, Portuguese propolis (from Populus sp.), Brazilian red propolis (from Dalbergia ecastophyllum), and Brazilian green propolis (from Baccharis dracunculifolia) in order to evaluate the antifungal potential for its application as a preservative in food. For that, the chemical composition was characterized by LC/DAD/ESI-MSn. Also, the total phenolic content, flavonoids and antioxidant activity was evaluated through UV-Vis spectrophotometry. Concerning the antifungal properties, the values differed in relation to the types of fungi, to the inoculating concentration and mode of action. With this in mind, an analysis of their antifungal activities at two levels of concentration (0.5-1.5 g/L and 5-15 g/L) was previously evaluated in vitro, with the aim of selecting the best concentration of inhibition of the rotting diameter of two types of grapes, namely, red and white. Subsequently, different fungi were used to test the activity of the aforementioned propolis, namely, Alternaria sp., Botrytis cinerea, Cladosporium sp., Penicillium sp. 2, Aspergillus carbonarius MUM04.46, and Aspergillus carbonarius MUM04.52. This last experiment made it possible to measure the rotting diameter of each grape and to evaluate the antifungal capacity of each type of propolis, with the final aim of using it to design an effective product that can extend the shelf life of food. |
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| Autores principais: | Diallo, Moustapha |
| Assunto: | Propolis Antifungal activity Food preservative Grapes LC/DAD/ESI-MSn Antioxidant activity UV-Vis |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | Propolis is a resin made by honeybees from multiple plant sources surrounding the hive. Therefore, each type of propolis from different regions has intrinsic characteristics related to their chemical composition, such as antifungal, antioxidative properties, and extrinsic such as color, both specific to their botanical origin. Thus, propolis can be regarded as a functional food with preservative properties offering an alternative to the concern of the global and growing use of synthetic preservatives considerably harming human health. The aim of this work was to study three types of propolis, namely, Portuguese propolis (from Populus sp.), Brazilian red propolis (from Dalbergia ecastophyllum), and Brazilian green propolis (from Baccharis dracunculifolia) in order to evaluate the antifungal potential for its application as a preservative in food. For that, the chemical composition was characterized by LC/DAD/ESI-MSn. Also, the total phenolic content, flavonoids and antioxidant activity was evaluated through UV-Vis spectrophotometry. Concerning the antifungal properties, the values differed in relation to the types of fungi, to the inoculating concentration and mode of action. With this in mind, an analysis of their antifungal activities at two levels of concentration (0.5-1.5 g/L and 5-15 g/L) was previously evaluated in vitro, with the aim of selecting the best concentration of inhibition of the rotting diameter of two types of grapes, namely, red and white. Subsequently, different fungi were used to test the activity of the aforementioned propolis, namely, Alternaria sp., Botrytis cinerea, Cladosporium sp., Penicillium sp. 2, Aspergillus carbonarius MUM04.46, and Aspergillus carbonarius MUM04.52. This last experiment made it possible to measure the rotting diameter of each grape and to evaluate the antifungal capacity of each type of propolis, with the final aim of using it to design an effective product that can extend the shelf life of food. |
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