Publicação
Differentiation of bee pollen origin through phenolic and volatile profiles
| Resumo: | Nowadays, bee products are notably commended by consumers for medicinal and dietary purposes. Bee pollen is particularly used as a precious source of nutritious compounds like proteins (which includes enzymes), carbohydrates, lipids, minerals as well as a large range of secondary plant metabolites (polyphenols, carotenoids, tocopherol and vitamins). According to the literature, bee pollen possesses great potentialities such as antioxidant, anti-inflammatory, antibacterial activities, among other . Therefore, it can be used as a food supplement to promote the human body resistance against numerous diseases including cancer. However, the bee pollen composition varies depending on the botanical and the geographical origin, which represents the major difficulty in the application of this matrix. The aim of the present study was to differentiate bee pollen origin through the phenolic and volatile profiles of samples from Portugal and Morocco. Also, the contribution of their phenolic compounds to the antioxidant and cytotoxic activities were investigated. After the determination of the total phenolics and flavonoid content analysis through UV-Vis analysis, the phenolic composition was assessed by liquid chromatography coupled to mass spectrometry (LC-MS). The volatile profile of the samples was access through gas chromatography coupled to mass spectrometry (GC-MS) after solid-phase microextraction (SPME). The antioxidant properties were measured by DPPH and ABTS scavenging activity and reducing power assays. Finally, the cytotoxicity of the extracts was examined employing different human tumor cell lines. Our results showed that bee pollen has a rich phenolic profile. Samples from Portugal displayed diverse phenylamides and flavonoid glycosides, while the Moroccan ones presented mostly flavonoid glycosides, phenylamides and some phenolic acids, except M7 which contained important phenylamide quantity. Concerning volatile compounds, plenty of compounds were detected majorly aldehydes, alcohols and some ketones. Some samples presented a high antioxidant capacity particularly the chestnut bee pollen from Bragança. In terms of anti-carcinogenic activity, only P1, Asteraceae carduus bee pollen, exhibited a remarkable anti-tumor potential against MCF-7 (breast carcinoma). Overall, Bee pollen samples belonging to the same floral origin presented similarities in both phenolic and volatile compounds profiles. Also, some detected phenolic and volatile compounds can be considered as fliral and geographical biomarkers. |
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| Autores principais: | Larbi, Samar |
| Assunto: | Bee pollen Phenolic compounds Volatile compounds Antioxidant activity Cytotoxicity activity |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | Nowadays, bee products are notably commended by consumers for medicinal and dietary purposes. Bee pollen is particularly used as a precious source of nutritious compounds like proteins (which includes enzymes), carbohydrates, lipids, minerals as well as a large range of secondary plant metabolites (polyphenols, carotenoids, tocopherol and vitamins). According to the literature, bee pollen possesses great potentialities such as antioxidant, anti-inflammatory, antibacterial activities, among other . Therefore, it can be used as a food supplement to promote the human body resistance against numerous diseases including cancer. However, the bee pollen composition varies depending on the botanical and the geographical origin, which represents the major difficulty in the application of this matrix. The aim of the present study was to differentiate bee pollen origin through the phenolic and volatile profiles of samples from Portugal and Morocco. Also, the contribution of their phenolic compounds to the antioxidant and cytotoxic activities were investigated. After the determination of the total phenolics and flavonoid content analysis through UV-Vis analysis, the phenolic composition was assessed by liquid chromatography coupled to mass spectrometry (LC-MS). The volatile profile of the samples was access through gas chromatography coupled to mass spectrometry (GC-MS) after solid-phase microextraction (SPME). The antioxidant properties were measured by DPPH and ABTS scavenging activity and reducing power assays. Finally, the cytotoxicity of the extracts was examined employing different human tumor cell lines. Our results showed that bee pollen has a rich phenolic profile. Samples from Portugal displayed diverse phenylamides and flavonoid glycosides, while the Moroccan ones presented mostly flavonoid glycosides, phenylamides and some phenolic acids, except M7 which contained important phenylamide quantity. Concerning volatile compounds, plenty of compounds were detected majorly aldehydes, alcohols and some ketones. Some samples presented a high antioxidant capacity particularly the chestnut bee pollen from Bragança. In terms of anti-carcinogenic activity, only P1, Asteraceae carduus bee pollen, exhibited a remarkable anti-tumor potential against MCF-7 (breast carcinoma). Overall, Bee pollen samples belonging to the same floral origin presented similarities in both phenolic and volatile compounds profiles. Also, some detected phenolic and volatile compounds can be considered as fliral and geographical biomarkers. |
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