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Phenolic profile of hydromethanolic extract and decoction of Rubus ulmifolius Schott

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Detalhes bibliográficos
Resumo:Rubus ulmifolius Schoot (Rosaceae), known as wild blackberry, is a perennial shrubfound in wild and cultivated habitats in Europe, Asia and North Africa[1]. Traditionally, R. ulmifoliusis considered to be anticatarrhal, antiseptic, diuretic, anti-inflammatory, antioxidant, astringent, and antispasmodic. Oral dosage forms are used for diarrhea, menstrual pain, menopause disorders, liver diseases, aphtha, gingivitis, hypertension and diabetes [2].Different classes of bioactive constituents are present in R. ulmifolius, including phenolic compounds. This study aimed to determine the phenolic composition of different preparations (decoction and hydromethanolic extract)of R. ulmifoliusflower buds. The analysis of phenolic compounds was carried out by reversed-phase high performance liquid chromatography coupled to diode array detection and mass spectrometry with electron spray ionization (HPLC-DAD-ESI/MS), in order to establish the specific phenolic profile of each preparation. The phenolic profile of both preparations was identical, varying only in the concentrations found. Twenty-three phenolic compounds were identified in both samples, in which seven were identified as phenolic acids (di- and caffeolyqui nic, p-coumaroylquinicand feruloylquinic acids),ten flavonoids (quercetin and kaempferol derivatives and catechin), and five ellagitannin derivatives (lambertianin and di -HHDP-galloyl glucose). These compoundswere found in both preparations. The most abundant compounds foundwere ellagitannin derivatives, such as lambertianin and di-HHDP-galloyl glucose. Gasperotti et al. [3]have identified these compounds in R. fruticosus fruits. To our knowledge, this is the first time that these compounds were identified and quantified inR. ulmifolius flowers buds.
Autores principais:Martins, Ana
Outros Autores:Barros, Lillian; Dueñas, Montserrat; Carvalho, Ana Maria; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Ano:2013
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
Descrição
Resumo:Rubus ulmifolius Schoot (Rosaceae), known as wild blackberry, is a perennial shrubfound in wild and cultivated habitats in Europe, Asia and North Africa[1]. Traditionally, R. ulmifoliusis considered to be anticatarrhal, antiseptic, diuretic, anti-inflammatory, antioxidant, astringent, and antispasmodic. Oral dosage forms are used for diarrhea, menstrual pain, menopause disorders, liver diseases, aphtha, gingivitis, hypertension and diabetes [2].Different classes of bioactive constituents are present in R. ulmifolius, including phenolic compounds. This study aimed to determine the phenolic composition of different preparations (decoction and hydromethanolic extract)of R. ulmifoliusflower buds. The analysis of phenolic compounds was carried out by reversed-phase high performance liquid chromatography coupled to diode array detection and mass spectrometry with electron spray ionization (HPLC-DAD-ESI/MS), in order to establish the specific phenolic profile of each preparation. The phenolic profile of both preparations was identical, varying only in the concentrations found. Twenty-three phenolic compounds were identified in both samples, in which seven were identified as phenolic acids (di- and caffeolyqui nic, p-coumaroylquinicand feruloylquinic acids),ten flavonoids (quercetin and kaempferol derivatives and catechin), and five ellagitannin derivatives (lambertianin and di -HHDP-galloyl glucose). These compoundswere found in both preparations. The most abundant compounds foundwere ellagitannin derivatives, such as lambertianin and di-HHDP-galloyl glucose. Gasperotti et al. [3]have identified these compounds in R. fruticosus fruits. To our knowledge, this is the first time that these compounds were identified and quantified inR. ulmifolius flowers buds.