Publicação
Effects of Cryphonectria parasitica infection by CHV1 hypovirus in the pathogenesis‐related OAH activity
| Resumo: | Cryphonectria parasitica, the causal agent of chestnut blight, causes necrotic lesions (cankers) on the bark of stems and branches of susceptible host trees leading to wilting of the plant part distal to the infection. Cryphonectria hypovirus 1 (CHV1) infects C. parasitica and reduces the fungus virulence (hypovirulence). The interaction between C. parasitica and CHV1 has been studied intensively at the molecular level. In addition to the reduced virulence the CHV1 infected strains also display other phenotypic symptoms, including reduced pigmentation and sporulation. It has been shown that CHV1 generates these phenotypic changes by interfering with parasitic sign transduction pathways. After infection and mycelium spread, wound periderm formation is continuously inhibited in susceptible chestnut species because the advancing mycelial fans stop the host cells by means of toxins and cell wall‐degrading enzymes. The oxalic acid is one of the metabolites which is associated in this process. It has a toxic effect on host cells and is creted by C. parasitica at the advancing edge of the infection. The objectives of this work were to evaluate the oxalic acid production, in microbiological culture medium of wild type virulent C. parasitica and in hypovirulent isogenic strains. The evaluation of the virulence of C. parasitica, virulent and hypovirulent isolates was performed by inoculation in young branches of C. sativa. This method allows us to characterize the degree of virulence of six virulent isolates; Cast13, VBC02, Cast26, Cast13, Cast17, Cast 07, VDP 11 and hypovirulent converted by RBB 111. Determination of oxalic acid by spectrophotometry allows us to evaluate production of oxalic acid in each virulent isolate and their converted ones. |
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| Autores principais: | Gadoum, Horiya |
| Assunto: | Chestnut blight Cryphonectria parasitica Cryphonectria hypovirus 1 Hypovirulence Oxaloacetate acethylhydrolase activity Oxalic acid |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | Cryphonectria parasitica, the causal agent of chestnut blight, causes necrotic lesions (cankers) on the bark of stems and branches of susceptible host trees leading to wilting of the plant part distal to the infection. Cryphonectria hypovirus 1 (CHV1) infects C. parasitica and reduces the fungus virulence (hypovirulence). The interaction between C. parasitica and CHV1 has been studied intensively at the molecular level. In addition to the reduced virulence the CHV1 infected strains also display other phenotypic symptoms, including reduced pigmentation and sporulation. It has been shown that CHV1 generates these phenotypic changes by interfering with parasitic sign transduction pathways. After infection and mycelium spread, wound periderm formation is continuously inhibited in susceptible chestnut species because the advancing mycelial fans stop the host cells by means of toxins and cell wall‐degrading enzymes. The oxalic acid is one of the metabolites which is associated in this process. It has a toxic effect on host cells and is creted by C. parasitica at the advancing edge of the infection. The objectives of this work were to evaluate the oxalic acid production, in microbiological culture medium of wild type virulent C. parasitica and in hypovirulent isogenic strains. The evaluation of the virulence of C. parasitica, virulent and hypovirulent isolates was performed by inoculation in young branches of C. sativa. This method allows us to characterize the degree of virulence of six virulent isolates; Cast13, VBC02, Cast26, Cast13, Cast17, Cast 07, VDP 11 and hypovirulent converted by RBB 111. Determination of oxalic acid by spectrophotometry allows us to evaluate production of oxalic acid in each virulent isolate and their converted ones. |
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