Publicação
Tolerance and stress response of Macrolepiota procera to nickel
| Resumo: | Nickel (Ni) is an essential element for many organisms; however, it is very toxic at high concentrations and also depending on the species. In macrofungi the mechanisms underlying their Ni tolerance are poorly documented. This study examines, for the first time, the participation of the antioxidative system in Macrolepiota procera exposed to different Ni2+ concentrations and their relation with Ni tolerance. The effect of the pH on Ni tolerance was also evaluated. The fungus was cultivated on solid medium with different NiCl2 concentrations (0.05, 0.2, 0.8 mM) at pH 4, 6, and 8, and fungi growth and Ni uptake were determined. The antioxidative enzymes catalase (CAT) and superoxide dismutase (SOD) and the production of hydrogen peroxide (H2O2) were evaluated on fungal submerged cultures within the first hours of Ni2+ exposure. Results showed that M. procera growth decreased when Ni2+ concentrations increased, reaching a maximum growth inhibition (>80%) up to 0.2 mM of NiCl2. Ni uptake increased proportionally to Ni increase in the medium. Both Ni tolerance and Ni accumulation were affected by medium pH. Microscope observations showed differences in the size of spores produced by fungi at different Ni concentrations. Ni exposure induced oxidative stress, as indicated by the production of H2O2, the levels of which seem to be regulated by the antioxidant enzymes SOD and CAT. The time variation pattern of SOD and CAT activities indicated that the former has a greater role in alleviating the stress. The results obtained suggested that tolerance of M. procera to Ni2+ is associated with the ability of this macrofungus to initiate an efficient antioxidant defense system |
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| Autores principais: | Baptista, Paula |
| Outros Autores: | Ferreira, Sílvia; Soares, Maria Elisa; Coelho, Valentim; Bastos, Maria de Lourdes |
| Assunto: | Macrolepiota procera Nickel Stress Ni2+ tolerance Ni2+ accumulation Hydrogen peroxide Antioxidant enzymes |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | Nickel (Ni) is an essential element for many organisms; however, it is very toxic at high concentrations and also depending on the species. In macrofungi the mechanisms underlying their Ni tolerance are poorly documented. This study examines, for the first time, the participation of the antioxidative system in Macrolepiota procera exposed to different Ni2+ concentrations and their relation with Ni tolerance. The effect of the pH on Ni tolerance was also evaluated. The fungus was cultivated on solid medium with different NiCl2 concentrations (0.05, 0.2, 0.8 mM) at pH 4, 6, and 8, and fungi growth and Ni uptake were determined. The antioxidative enzymes catalase (CAT) and superoxide dismutase (SOD) and the production of hydrogen peroxide (H2O2) were evaluated on fungal submerged cultures within the first hours of Ni2+ exposure. Results showed that M. procera growth decreased when Ni2+ concentrations increased, reaching a maximum growth inhibition (>80%) up to 0.2 mM of NiCl2. Ni uptake increased proportionally to Ni increase in the medium. Both Ni tolerance and Ni accumulation were affected by medium pH. Microscope observations showed differences in the size of spores produced by fungi at different Ni concentrations. Ni exposure induced oxidative stress, as indicated by the production of H2O2, the levels of which seem to be regulated by the antioxidant enzymes SOD and CAT. The time variation pattern of SOD and CAT activities indicated that the former has a greater role in alleviating the stress. The results obtained suggested that tolerance of M. procera to Ni2+ is associated with the ability of this macrofungus to initiate an efficient antioxidant defense system |
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