Publicação
Efect of Gamma irradiation on the microbiological quality of dehydrated bee-pollen
| Resumo: | The objective of this study was to determinate some microbiological parameters of seven dehydrated bee-pollen collected from September to December of2012, in Brazil, before and after irradiation, in order to observe if the conservation process influenced the final microbiological load. All bee-pollen samples were collected in the same apiary and processed as usual; then one parcel of each one was gamma-irradiated to achieve dose of 5.0 kGy with a cobalt-60 source (by EMBRARAD/Empresa Brasileira de Radia~aes Ltda, Cotia, SAo Paulo, Brasil). The bee-pollen samples were analysed to enumerate aerobic mesophilic and psychotrophs bacteria, Staphylococcus aureus, total coliforms and yeasts and molds and to determine the presence or absent of sulphite-reducing Clostridium spores and Salmonella. Each sample ( 10 g) was homogenised and serial dilutions were inoculated on specific medium. Afterwards, incubations were performed depending on the microorganism (Estevinho et al., 2012). Results were expressed as colonyforming units per gram of dehydrated bee-pollen (CFU.g-1) for mesophilic, psychotrophs, Staphylococcus aureus, total coliforms and yeasts and molds or as presence or absent for sulphite-reducing Clostridium spores and Salmonella. |
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| Autores principais: | De-Melo, Adriane A.M. |
| Outros Autores: | Estevinho, Leticia M.; Almeida-Muradian, Ligia Bicudo |
| Assunto: | Bee-pollen Gamma-irradiation Microbiological parameters |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | The objective of this study was to determinate some microbiological parameters of seven dehydrated bee-pollen collected from September to December of2012, in Brazil, before and after irradiation, in order to observe if the conservation process influenced the final microbiological load. All bee-pollen samples were collected in the same apiary and processed as usual; then one parcel of each one was gamma-irradiated to achieve dose of 5.0 kGy with a cobalt-60 source (by EMBRARAD/Empresa Brasileira de Radia~aes Ltda, Cotia, SAo Paulo, Brasil). The bee-pollen samples were analysed to enumerate aerobic mesophilic and psychotrophs bacteria, Staphylococcus aureus, total coliforms and yeasts and molds and to determine the presence or absent of sulphite-reducing Clostridium spores and Salmonella. Each sample ( 10 g) was homogenised and serial dilutions were inoculated on specific medium. Afterwards, incubations were performed depending on the microorganism (Estevinho et al., 2012). Results were expressed as colonyforming units per gram of dehydrated bee-pollen (CFU.g-1) for mesophilic, psychotrophs, Staphylococcus aureus, total coliforms and yeasts and molds or as presence or absent for sulphite-reducing Clostridium spores and Salmonella. |
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