Publicação
Antibiotic-Resistant Escherichia coli on Asian clam (Bivalvia, Corbiculidea): Case Study in the Tua River, Portugal.
| Resumo: | Antimicrobial resistance (AMR) is a major global health problem. Water use by human activities represent a natural reservoir of AMR, thus a time-integrated approach is required in aquatic ecosystems. Freshwater bivalves are widely applied as accumulation indicators and monitors of contaminant effects on different levels of biological integration once they are suspension feeders that actively filter, retain and concentrates particles from their surrounding water, including free living or particle-bound bacteria. In this study, we aimed to (i) investigate the genetic diversity of E. coli, (ii) determine the virulence factors of E. coli isolates, and (iii) detect antibiotic resistant E. coli. Samples were collected in the middle sector of the Tua River. Isolates were retrieved in Chromocult® Coliform Agar plates (Merck, Germany). Susceptibility testing was performed by the disc diffusion method, according to the Clinical Laboratory Standards Institute (CLSI), and the screening of phenotypic ESBL production was carried out by the double disk synergy test using cefotaxime (30μg), ceftazidime (30μg), and amoxicillin/clavulanate (20/10μg) acid discs. PCR-based assays were designed for phylogenetic E. coli groups evaluation, resistance and detection of ESBL genes. The E. coli phylogenetic group B1 was the most prevalent (44 %), followed by phylogenetic groups A and D. Phylogenetic groups A and B1 comprised more than half (70 %) of the total E. coli isolated. Our results show that Corbicula spp. provide an efficient, time-integrating tool for identifying/quantifying faecal indicators, including multidrug resistant bacteria. |
|---|---|
| Autores principais: | Saavedra, Maria José |
| Outros Autores: | Gomes, Sónia; Fernandes, Conceição; Teixeira, Amílcar; Varandas, Simone |
| Assunto: | E. coli Antimicrobial resistance Corbicula fluminea |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | Antimicrobial resistance (AMR) is a major global health problem. Water use by human activities represent a natural reservoir of AMR, thus a time-integrated approach is required in aquatic ecosystems. Freshwater bivalves are widely applied as accumulation indicators and monitors of contaminant effects on different levels of biological integration once they are suspension feeders that actively filter, retain and concentrates particles from their surrounding water, including free living or particle-bound bacteria. In this study, we aimed to (i) investigate the genetic diversity of E. coli, (ii) determine the virulence factors of E. coli isolates, and (iii) detect antibiotic resistant E. coli. Samples were collected in the middle sector of the Tua River. Isolates were retrieved in Chromocult® Coliform Agar plates (Merck, Germany). Susceptibility testing was performed by the disc diffusion method, according to the Clinical Laboratory Standards Institute (CLSI), and the screening of phenotypic ESBL production was carried out by the double disk synergy test using cefotaxime (30μg), ceftazidime (30μg), and amoxicillin/clavulanate (20/10μg) acid discs. PCR-based assays were designed for phylogenetic E. coli groups evaluation, resistance and detection of ESBL genes. The E. coli phylogenetic group B1 was the most prevalent (44 %), followed by phylogenetic groups A and D. Phylogenetic groups A and B1 comprised more than half (70 %) of the total E. coli isolated. Our results show that Corbicula spp. provide an efficient, time-integrating tool for identifying/quantifying faecal indicators, including multidrug resistant bacteria. |
|---|