Publicação
Selection of glyphosate-degrading microorganisms isolated from vineyard soils
| Resumo: | Glyphosate is the most widely used herbicide worldwide. It is a non-selective, systemic, post-emergence herbicide that controls more weed species than any other. Its extensively use have been led to the contamination of both terrestrial and aquatic ecosystems, becoming a huge environmental concern worldwide. Bioremediation using glyphosate-degrading microorganisms could be a promising approach to overcome the risks associated to the accumulation of this herbicide in the environment. Hence, this work aims to isolate bacteria and fungi from vineyard soil's exposed to glyphosate and identify potential strains for bioremediation of glyphosate-polluted soils. Accordingly, microorganisms were isolated from soils collected in three different slope positions (top, middle and bottom), and were further screened for their ability to grow in medium containing glyphosate as the sole phosphorus source. Overall, 477 glyphosate-tolerant morphotypes (325 bacteria and 152 fungi) were isolated from vineyard soil’s. Slope position showed to influenced significantly the diversity of bacteria but also some soil physicochemical properties. Contents on Extractable phosphorus (E.P.) in soils affected significantly the composition of the whole microbial community, in particular of bacteria. Among the strains screened, 55% of the bacteria and 33 % of the fungi showed the capacity to utilize glyphosate efficiently. The sequencing of the 16S (for bacteria) and ITS (for fungi) region of rRNA, identified Erwinia billingiae (strain B105), Pseudomonas fragi (strains B328 and B295), Pseudomonas sp. 1 (strain B93) and Trichoderma sp. 2 (strain F54), as the best potential candidates for bioremediation of glyphosate-polluted soils. Their bioremediation effectiveness must be evaluated in future studies. |
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| Autores principais: | Nunes, Cátia Fernandes |
| Assunto: | Herbicide Fungi Bacteria Biodegradation |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | Glyphosate is the most widely used herbicide worldwide. It is a non-selective, systemic, post-emergence herbicide that controls more weed species than any other. Its extensively use have been led to the contamination of both terrestrial and aquatic ecosystems, becoming a huge environmental concern worldwide. Bioremediation using glyphosate-degrading microorganisms could be a promising approach to overcome the risks associated to the accumulation of this herbicide in the environment. Hence, this work aims to isolate bacteria and fungi from vineyard soil's exposed to glyphosate and identify potential strains for bioremediation of glyphosate-polluted soils. Accordingly, microorganisms were isolated from soils collected in three different slope positions (top, middle and bottom), and were further screened for their ability to grow in medium containing glyphosate as the sole phosphorus source. Overall, 477 glyphosate-tolerant morphotypes (325 bacteria and 152 fungi) were isolated from vineyard soil’s. Slope position showed to influenced significantly the diversity of bacteria but also some soil physicochemical properties. Contents on Extractable phosphorus (E.P.) in soils affected significantly the composition of the whole microbial community, in particular of bacteria. Among the strains screened, 55% of the bacteria and 33 % of the fungi showed the capacity to utilize glyphosate efficiently. The sequencing of the 16S (for bacteria) and ITS (for fungi) region of rRNA, identified Erwinia billingiae (strain B105), Pseudomonas fragi (strains B328 and B295), Pseudomonas sp. 1 (strain B93) and Trichoderma sp. 2 (strain F54), as the best potential candidates for bioremediation of glyphosate-polluted soils. Their bioremediation effectiveness must be evaluated in future studies. |
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