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Measurement of multi-red blood cells interactions in blood flow by confocal micro-PTV

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Detalhes bibliográficos
Resumo:In microcirculation the flow behavior of red blood cells (RBCs) plays a crucial role in many physiological and pathological phenomena. For instance, the interaction of RBCs in shear flow is believed to play an important role to the thrombogenesis process. Despite the relevance of this phenomenon on the blood mass transport, very little studies have been performed during the years, partly due to the absence of adequate visualization techniques able to obtain both direct and quantitative measurements on multi-RBCs motions in concentrated suspensions. Past studies on both individual and concentrated RBCs used conventional microscopes and/or ghost cells to obtain visible trace RBCs at high concentration suspension of blood cells [1, 2]. Recently, advances of confocal microscopy and consequent advantages over conventional microscopes have led to an emerging technique known as confocal micro-PIV [3, 4]. This paper presents the application of a confocal micro- PTV system to measure RBC-RBC hydrodynamic interactions in flowing blood.
Autores principais:Lima, Rui A.
Outros Autores:Ishikawa, Takuji; Fujiwara, Hiroki; Takeda, Motohiro; Imai, Yohsuke; Tsubota, Ken-ichi; Matsuki, Noriaki; Wada, Shigeo; Yamaguchi, Takami
Assunto:Red blood cell Microcirculation
Ano:2007
País:Portugal
Tipo de documento:comunicação em conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
Descrição
Resumo:In microcirculation the flow behavior of red blood cells (RBCs) plays a crucial role in many physiological and pathological phenomena. For instance, the interaction of RBCs in shear flow is believed to play an important role to the thrombogenesis process. Despite the relevance of this phenomenon on the blood mass transport, very little studies have been performed during the years, partly due to the absence of adequate visualization techniques able to obtain both direct and quantitative measurements on multi-RBCs motions in concentrated suspensions. Past studies on both individual and concentrated RBCs used conventional microscopes and/or ghost cells to obtain visible trace RBCs at high concentration suspension of blood cells [1, 2]. Recently, advances of confocal microscopy and consequent advantages over conventional microscopes have led to an emerging technique known as confocal micro-PIV [3, 4]. This paper presents the application of a confocal micro- PTV system to measure RBC-RBC hydrodynamic interactions in flowing blood.