Publicação
Assessment of bacterial cellulose biosynthesis from olive bagasse using photostimulation
| Resumo: | The biosynthesis of bacterial cellulose (BC) is an innovative approach with a variety of applications. Strategies have been studied to economically enable the process, with the most common being research on fermentation media to overcome limitations and increase competitiveness in the market, thus allowing greater diversity in the use of this biopolymer. Moist olive pomace (MOP) is a byproduct of the olive oil industry that can be phytotoxic to soil and water bodies, potentially causing environmental impact. This study aimed to evaluate BC production with various concentrations of MOP (1%, 5%, 10%, 20%, 30%, and 40%) and analyze the effect of LED irradiation at 630 ± 10 nm, depositing an energy density of 14 J/cm². Komagataeibacter intermedius SB14, a cellulose-producing strain, was identified and studied for BC production. The characterization of cellulose membranes was performed using Fourier- transform infrared spectroscopy (FTIR), thermogravimetric analysis, mechanical testing, and evaluation of antioxidant and antimicrobial activity. A significant increase (p < 0.0001) in BC production was observed in the 20% MOP group, with a 166.95% increase compared to the control (0% MOP). The result indicated a cellular response due to stress caused by the phenolic compounds present, leading to increased BC production. LED light emission led to a significant increase in BC production, with 124% in the control group and 392% in the 20% MOP group. Characteristic cellulose groups were identified in all produced samples. Mechanical testing revealed greater tensile strength and deformation capacity of the cellulose membrane produced with 20% MOP (60.73 MPa and 0.696 mm/mm) compared to the control (12.75 MPa and 0.098 mm/mm), respectively. Although increased antioxidant activity was observed in the MOP- containing medium, no antimicrobial activity was detected in any of the produced samples. This work highlights a sustainable pathway by introducing MOP into the circular economy chain and leveraging LED irradiation in the bioprocess to enhance production and reduce costs. |
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| Autores principais: | Khelifa, Halima |
| Assunto: | Biopolymer Komagataeibacter intermedius Moist olive pomace LED irradiation |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| Resumo: | The biosynthesis of bacterial cellulose (BC) is an innovative approach with a variety of applications. Strategies have been studied to economically enable the process, with the most common being research on fermentation media to overcome limitations and increase competitiveness in the market, thus allowing greater diversity in the use of this biopolymer. Moist olive pomace (MOP) is a byproduct of the olive oil industry that can be phytotoxic to soil and water bodies, potentially causing environmental impact. This study aimed to evaluate BC production with various concentrations of MOP (1%, 5%, 10%, 20%, 30%, and 40%) and analyze the effect of LED irradiation at 630 ± 10 nm, depositing an energy density of 14 J/cm². Komagataeibacter intermedius SB14, a cellulose-producing strain, was identified and studied for BC production. The characterization of cellulose membranes was performed using Fourier- transform infrared spectroscopy (FTIR), thermogravimetric analysis, mechanical testing, and evaluation of antioxidant and antimicrobial activity. A significant increase (p < 0.0001) in BC production was observed in the 20% MOP group, with a 166.95% increase compared to the control (0% MOP). The result indicated a cellular response due to stress caused by the phenolic compounds present, leading to increased BC production. LED light emission led to a significant increase in BC production, with 124% in the control group and 392% in the 20% MOP group. Characteristic cellulose groups were identified in all produced samples. Mechanical testing revealed greater tensile strength and deformation capacity of the cellulose membrane produced with 20% MOP (60.73 MPa and 0.696 mm/mm) compared to the control (12.75 MPa and 0.098 mm/mm), respectively. Although increased antioxidant activity was observed in the MOP- containing medium, no antimicrobial activity was detected in any of the produced samples. This work highlights a sustainable pathway by introducing MOP into the circular economy chain and leveraging LED irradiation in the bioprocess to enhance production and reduce costs. |
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