Publicação

Optimization of the aptamers’ immobilization conditions for maximizing the response of a dualaptasensor for cancer biomarker detection

Ver documento

Detalhes bibliográficos
Resumo:A simple and sensitive method that allows the simultaneous detection of single or multiple cancer biomarkers is envisaged and may be an important tool in cancer diagnosis. Osteopontin (OPN) is a protein that is present in several body fluids and has been reported as a possible cancer biomarker. The voltammetric signals generated by the dual-aptasensor array, after the formation of the aptamers-protein complex, were monitored using cyclic voltammetry (CV) and square-wave voltammetry (SWV), using [Fe(CN)6] 3/ 4 as a redox probe. The aim of this work is to optimize the experimental conditions to development a label-free voltammetric dual-aptasensor array for the detection of human osteopontin using a 2k factorial experimental design.
Autores principais:Meirinho, Sofia G.
Outros Autores:Ezziddine, Maha; Veloso, Ana C.A.; Dias, L.G.; Rodrigues, Lígia R.; Othmane, Ali; Peres, António M.
Ano:2017
País:Portugal
Tipo de documento:póster em conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
Descrição
Resumo:A simple and sensitive method that allows the simultaneous detection of single or multiple cancer biomarkers is envisaged and may be an important tool in cancer diagnosis. Osteopontin (OPN) is a protein that is present in several body fluids and has been reported as a possible cancer biomarker. The voltammetric signals generated by the dual-aptasensor array, after the formation of the aptamers-protein complex, were monitored using cyclic voltammetry (CV) and square-wave voltammetry (SWV), using [Fe(CN)6] 3/ 4 as a redox probe. The aim of this work is to optimize the experimental conditions to development a label-free voltammetric dual-aptasensor array for the detection of human osteopontin using a 2k factorial experimental design.