Publicação
Development of a Bi-layer Hydrogel via 3D Printing for articular cartilage replacement: PVA/Chitosan/HAp/CNF Scaffold with a PVA/Tannic Acid Surface
| Resumo: | This investigation develops and characterizes a bi-layer hydrogel system designed for articular cartilage regeneration, combining a robocasted PVA scaffold reinforced with hydroxyapatite, chitosan, and cellulose nanofibers, with an upper PVA/tannic acid layer engineered to optimize lubrication and bioadhesion. The paste formulation enabled a pseudoplastic rheological behaviour suitable for 3D printing, resulting in mechanically stable porous structures with controlled geometry. The upper layer exhibited homogeneous surfaces, a low coefficient of friction, and effective integration with the scaffold, forming a continuous hybrid construct. Mechanical, tribological, chemical, and swelling analyses demonstrated that the bi-layer architecture simultaneously enhances strength, stability, and lubricating performance. Biological studies confirmed high cell viability and adequate adhesion in the irradiated versions. Overall, the results validate the potential of this system as a promising platform for cartilage replacement and regeneration, highlighting the role of polymeric combinations and additive manufacturing in tissue engineering. |
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| Autores principais: | Pinto, Fábio Alexandre Mouzinho de Sousa |
| Assunto: | Bi-layer hydrogel Articular cartilage regeneration Robocasting PVA-based composites Tannic acid lubrication Additive manufacturing Hidrogel bi-camada Regeneração de cartilagem articular Compósitos à base de PVA Lubrificação com ácido tânico Manufatura aditiva |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Setúbal |
| Idioma: | inglês |
| Origem: | Instituto Politécnico de Setúbal |
| Resumo: | This investigation develops and characterizes a bi-layer hydrogel system designed for articular cartilage regeneration, combining a robocasted PVA scaffold reinforced with hydroxyapatite, chitosan, and cellulose nanofibers, with an upper PVA/tannic acid layer engineered to optimize lubrication and bioadhesion. The paste formulation enabled a pseudoplastic rheological behaviour suitable for 3D printing, resulting in mechanically stable porous structures with controlled geometry. The upper layer exhibited homogeneous surfaces, a low coefficient of friction, and effective integration with the scaffold, forming a continuous hybrid construct. Mechanical, tribological, chemical, and swelling analyses demonstrated that the bi-layer architecture simultaneously enhances strength, stability, and lubricating performance. Biological studies confirmed high cell viability and adequate adhesion in the irradiated versions. Overall, the results validate the potential of this system as a promising platform for cartilage replacement and regeneration, highlighting the role of polymeric combinations and additive manufacturing in tissue engineering. |
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