Detalhes bibliográficos
| Resumo: | This note describes a rapid and inexpensive Restriction Fragment Length Polymorphism technique to discriminate all species of Atherina (Pisces: Atherinidae) of the north-eastern Atlantic and the Mediterranean. This technique is based on digestion of a fragment of the 12S ribosomal RNA (12SrRNA) gene region of mitochondrial DNA with restriction enzymes that recognize species-specific nucleotide sites. The three currently recognized species in the area, as well as two additional forms awaiting formal description, can be discriminated using a set of four endonucleases. We argue that this simple and fast technique may be of great help in the identification of young stages and in ecological surveys. |
|---|
| Autores principais: | Almada, Vítor Carvalho |
|---|
| Outros Autores: | Francisco, Sara Martins; Rosa, Inês; Domingues, Vera dos Santos; Cabral, Henrique; Almada, Frederico José Oliveira de; Henriques, Miguel; Robalo, Joana Isabel |
|---|
| Assunto: | Atherina RFLP mtDNA |
|---|
| Ano: | 2012 |
|---|
| País: | Portugal |
|---|
| Tipo de documento: | artigo |
|---|
| Tipo de acesso: | acesso aberto |
|---|
| Instituição associada: | Ispa-Instituto Universitário |
|---|
| Idioma: | inglês |
|---|
| Origem: | Repositório do Ispa - Instituto Universitário |
|---|