Publicação
Development of antimicrobial polysaccharide-based wound dressings
| Resumo: | Effective management of wounds is essential for preventing infections and promoting skin regeneration. Despite significant technological advances, many commercially available dressings exhibit limitations in exudate absorption, traumatic removal, and antimicrobial efficacy. In this context, polysaccharides have emerged as promising materials for the development of bioactive dressings, owing to their biocompatibility, biodegradability, and ability to form flexible films. This dissertation aimed to develop starch-based dressings plasticized with glycerol and doped with cationic porphyrins (TMePyP and TPPh3PyP), with the goal of combining suitable structural characteristics with antimicrobial photodynamic activity. The films were prepared by solvent casting and characterized in terms of physicochemical (thickness, wettability, residual moisture content, solubility, and singlet oxygen generation), mechanical (tensile strength, Young’s modulus, and elongation at break), and biological properties (antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa). Furthermore, the leaching behavior of the porphyrins in aqueous and biological media evaluated to support the interpretation of the biological performance results. The results obtained demonstrated that films doped with TMePyP exhibited greater flexibility, adequate wettability, and rapid release of the compound, suggesting potential applicability in the treatment of acute infections. Conversely, films with TPPh3PyP higher mechanical strength, lower solubility, and a more sustained release profile, features that may be advantageous for managing chronic infections. Films doped with porphyrins showed a high capacity for singlet oxygen generation under visible light irradiation and to significantly reduce bacterial viability in antimicrobial photodynamic therapy assays. These findings support the potential of these materials as promising photoactive platforms for antimicrobial wound dressings, adaptable to various clinical scenarios. |
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| Autores principais: | Trindade, Patrícia Rafael |
| Assunto: | Wound dressings Porphyrins Photodynamic therapy Starch films Antimicrobial materials Controlled release |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| Resumo: | Effective management of wounds is essential for preventing infections and promoting skin regeneration. Despite significant technological advances, many commercially available dressings exhibit limitations in exudate absorption, traumatic removal, and antimicrobial efficacy. In this context, polysaccharides have emerged as promising materials for the development of bioactive dressings, owing to their biocompatibility, biodegradability, and ability to form flexible films. This dissertation aimed to develop starch-based dressings plasticized with glycerol and doped with cationic porphyrins (TMePyP and TPPh3PyP), with the goal of combining suitable structural characteristics with antimicrobial photodynamic activity. The films were prepared by solvent casting and characterized in terms of physicochemical (thickness, wettability, residual moisture content, solubility, and singlet oxygen generation), mechanical (tensile strength, Young’s modulus, and elongation at break), and biological properties (antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa). Furthermore, the leaching behavior of the porphyrins in aqueous and biological media evaluated to support the interpretation of the biological performance results. The results obtained demonstrated that films doped with TMePyP exhibited greater flexibility, adequate wettability, and rapid release of the compound, suggesting potential applicability in the treatment of acute infections. Conversely, films with TPPh3PyP higher mechanical strength, lower solubility, and a more sustained release profile, features that may be advantageous for managing chronic infections. Films doped with porphyrins showed a high capacity for singlet oxygen generation under visible light irradiation and to significantly reduce bacterial viability in antimicrobial photodynamic therapy assays. These findings support the potential of these materials as promising photoactive platforms for antimicrobial wound dressings, adaptable to various clinical scenarios. |
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