Publicação
New molecular variants of epsilon and beta IncP-1 plasmids are present in estuarine waters
| Resumo: | In this work the presence of broad-host-plasmids in an estuary in Portugal has been inves- tigated. Pseudomonas putida KT2442 was used as model recipient bacteria in biparental matings with tetracycline and mercury to select for resistance phenotypes. As a result, 7 transconjugants were shown to carry broad-host-plasmids from the IncP-1 group, as seen by PCR amplification of the trfA gene. Sequence analysis confirmed the isolation of 4 plas- mids from b -1 subgroup and 3 assigned to the recently described e subgroup. To our knowledge this is the first report concerning the detection and isolation of IncP-1 b and e plasmids in estuarine waters. Moreover it is shown that, even though the retrieved plas- mids are phylogenetically close to previously characterized plasmids, such as pB10 and pKJK5, respectively, they constitute new molecular variants. |
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| Autores principais: | Oliveira, C. S. |
| Outros Autores: | Lázaro, B.; Azevedo, J. S. N.; Henriques, I.; Almeida, A.; Correia, A. |
| Assunto: | Estuary IncP-1 plasmid Broad-host-range-plasmid trfA gene Surface sea microlayer Ria de Aveiro |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| Resumo: | In this work the presence of broad-host-plasmids in an estuary in Portugal has been inves- tigated. Pseudomonas putida KT2442 was used as model recipient bacteria in biparental matings with tetracycline and mercury to select for resistance phenotypes. As a result, 7 transconjugants were shown to carry broad-host-plasmids from the IncP-1 group, as seen by PCR amplification of the trfA gene. Sequence analysis confirmed the isolation of 4 plas- mids from b -1 subgroup and 3 assigned to the recently described e subgroup. To our knowledge this is the first report concerning the detection and isolation of IncP-1 b and e plasmids in estuarine waters. Moreover it is shown that, even though the retrieved plas- mids are phylogenetically close to previously characterized plasmids, such as pB10 and pKJK5, respectively, they constitute new molecular variants. |
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