Publicação
Engineering off-the-shelf 3D in vitro models
| Resumo: | Cryopreservation is an essential technique for the short- and long-term preservation of biological tissues. Although this technique ensures global accessibility to these resources, it presents various challenges including the harmful physical-biochemical interface intrinsic to cryopreservation. From a medical perspective, and despite current developments in the field, organ cryopreservation remains unattainable. On the other hand, with the growing adoption of bioengineered in vitro models aimed at overcoming the problems of low correlation that animal models and two-dimensional surface cell culture present, in order to achieve a closer approximation of human (patho)physiology, there is also an increasing need to make such intrinsic testing platforms available to the entire scientific community. Cryopreservation serves, in this regard, as a solution for exporting these models to any infrastructure incapable of producing such platforms. Consequently, the growing demand for cryopreservable bioengineered models provides the ultimate purpose of this thesis through the optimization of an innovative formulation of cryoprotective agents for the cryopreservation of three-dimensional disease models containing cells and biomimetic components of the extracellular matrix. The results of cell viability and propagation after cryopreservation of these models thus demonstrate the success of the new cryopreservation formulation in protecting cells during 4 days of cryopreservation. It is hoped that the work carried out throughout this process will result in advances in the cryopreservation of 3D in vitro disease models that are suitable for export and ready for use anywhere in the world. |
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| Autores principais: | Borralho, Mafalda Saraiva |
| Assunto: | Cryopreservation Cryoprotectants Tissue engineering 3D disease models Pre-clinical analysis |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso embargado |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| Resumo: | Cryopreservation is an essential technique for the short- and long-term preservation of biological tissues. Although this technique ensures global accessibility to these resources, it presents various challenges including the harmful physical-biochemical interface intrinsic to cryopreservation. From a medical perspective, and despite current developments in the field, organ cryopreservation remains unattainable. On the other hand, with the growing adoption of bioengineered in vitro models aimed at overcoming the problems of low correlation that animal models and two-dimensional surface cell culture present, in order to achieve a closer approximation of human (patho)physiology, there is also an increasing need to make such intrinsic testing platforms available to the entire scientific community. Cryopreservation serves, in this regard, as a solution for exporting these models to any infrastructure incapable of producing such platforms. Consequently, the growing demand for cryopreservable bioengineered models provides the ultimate purpose of this thesis through the optimization of an innovative formulation of cryoprotective agents for the cryopreservation of three-dimensional disease models containing cells and biomimetic components of the extracellular matrix. The results of cell viability and propagation after cryopreservation of these models thus demonstrate the success of the new cryopreservation formulation in protecting cells during 4 days of cryopreservation. It is hoped that the work carried out throughout this process will result in advances in the cryopreservation of 3D in vitro disease models that are suitable for export and ready for use anywhere in the world. |
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