Publicação
Aqueous two-phase systems based on cholinium salts and tetrahydrofuran and their use for lipase purification
| Resumo: | Aqueous two-phase systems (ATPS) formed with cholinium-based ionic liquid - ILs (or salts) are a novel, low cost, and high efficient technique for the recovery of biomolecules. This study examines the formation of ATPS based on cholinium-based salts (cholinium chloride, cholinium bitartrate and cholinium dihydrogencitrate) and tetrahydrofuran (THF) for the purification of lipase from Bacillus sp. ITP-001, produced by submerged fermentation. The optimum conditions for this purification were determined to be 40 wt% of THF and 30 wt% of cholinium bitartrate at 25 degrees C. A purification factor of 130.1 +/- 11.7 fold, a lipase yield of 90.0 +/- 0.7% and a partition coefficient of enzyme for IL-rich phase (K-E = 0.11 +/- 0.01) and protein contaminants for THF-rich phase (K-P = 1.16 +/- 0.1) were achieved. (C) 2015 Elsevier B.V. All rights reserved. |
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| Autores principais: | Souza, Ranyere L. |
| Outros Autores: | Lima, Rafaella A.; Coutinho, Joao A. P.; Soares, Cleide M. F.; Lima, Alvaro S. |
| Assunto: | IONIC LIQUIDS BIPHASIC SYSTEMS POLYETHYLENE-GLYCOL PROTEIN SEPARATION ASPERGILLUS-NIGER MICROBIAL LIPASES EXTRACTION ECOTOXICITY BIODEGRADATION ACETONITRILE |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| Resumo: | Aqueous two-phase systems (ATPS) formed with cholinium-based ionic liquid - ILs (or salts) are a novel, low cost, and high efficient technique for the recovery of biomolecules. This study examines the formation of ATPS based on cholinium-based salts (cholinium chloride, cholinium bitartrate and cholinium dihydrogencitrate) and tetrahydrofuran (THF) for the purification of lipase from Bacillus sp. ITP-001, produced by submerged fermentation. The optimum conditions for this purification were determined to be 40 wt% of THF and 30 wt% of cholinium bitartrate at 25 degrees C. A purification factor of 130.1 +/- 11.7 fold, a lipase yield of 90.0 +/- 0.7% and a partition coefficient of enzyme for IL-rich phase (K-E = 0.11 +/- 0.01) and protein contaminants for THF-rich phase (K-P = 1.16 +/- 0.1) were achieved. (C) 2015 Elsevier B.V. All rights reserved. |
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