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Purification of plant complex protein extracts in non-denaturing conditions by in-solution isoelectric focusing
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| Resumo: | An alternative approach for plant complex protein extracts pre-purification by in-solution isoelectric focusing in non-denaturing conditions is presented. The separation of biologically active proteins, in narrow ranges of isoelectric point (pI) was obtained by a modified OFFGEL electrophoresis. Two different water-soluble protein extracts from Phragmites leaves were fractionated into 24 fractions within a 3-10 pI range at 10 °C in the absence of denaturing/reducing agents. One-dimensional electrophoretic analysis revealed different protein distribution patterns and the effective fractionation of both protein extracts. Peroxidase activity of each fraction confirmed that proteins remained active and pre-purification occurred. Biological triplicates assured the needed reproducibility. |
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| Autores principais: | Martins-Dias, Susete |
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| Outros Autores: | Ferreira, Renata |
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| Assunto: | Isoelectric focusing Plant extracts Plant leaves Plant proteins Poaceae |
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| Ano: | 2016 |
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| País: | Portugal |
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| Tipo de documento: | artigo |
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| Tipo de acesso: | acesso aberto |
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| Instituição associada: | Instituto Politécnico de Beja |
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| Idioma: | inglês |
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| Origem: | Repositório Institucional do IPBeja |
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