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Origin identification of maritime pine stands in France using chloroplast simple-sequence repeats

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Detalhes bibliográficos
Resumo:Maritime pine seed-lots from north-western Iberian regions (Portugal and Galicia) were introduced in the 1950s to the southwest of France (Aquitaine region), and the stands they formed suffered considerable frost damage. In the mid 1980s, a biochemical test was developed to test the putative origin of adult stands in Aquitaine, before seeds could be distributed for commercial purposes in France. In this paper, we describe a new test employing chloroplast simple-sequence repeats (cpSSRs) to facilitate identification of stand origin based on randomisation tests. The origin of five stands of unknown origin was determined with both the cpSSR and biochemical (terpene profile analysis) tests. The results from the two tests were concordant, but the DNA-based test gave faster and more accurate results. Use of this test should help when determining the origin of maritime pine stands in the Aquitaine region of France.
Autores principais:Ribeiro, M.M.A.
Outros Autores:Le Provost, Grégoire; Gerber, Sophie; Vendramin, Giovanni Giuseppe; Anzidei, Maria; Decroocq, Stéphane; Marpeau, Anne; Mariette, Stéphanie; Plomion, Christophe
Assunto:CPSSR Microsatellites Terpene Origin identification Pinus pinaster
Ano:2001
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Instituto Politécnico de Castelo Branco
Idioma:inglês
Origem:Repositório Científico do Instituto Politécnico de Castelo Branco
Descrição
Resumo:Maritime pine seed-lots from north-western Iberian regions (Portugal and Galicia) were introduced in the 1950s to the southwest of France (Aquitaine region), and the stands they formed suffered considerable frost damage. In the mid 1980s, a biochemical test was developed to test the putative origin of adult stands in Aquitaine, before seeds could be distributed for commercial purposes in France. In this paper, we describe a new test employing chloroplast simple-sequence repeats (cpSSRs) to facilitate identification of stand origin based on randomisation tests. The origin of five stands of unknown origin was determined with both the cpSSR and biochemical (terpene profile analysis) tests. The results from the two tests were concordant, but the DNA-based test gave faster and more accurate results. Use of this test should help when determining the origin of maritime pine stands in the Aquitaine region of France.