Publicação
Analysis of Aspergillus spp. burden by culture based-methods and molecular methods in different occupational environments: what needs to be done?
| Resumo: | Aim of the study - Fungal burden has traditionally been detected by conventional culture analysis, which despite its limitations, is widely used by the scientific community. Alternatively, quantitative real-time PCR (qPCR), based on the amplification of genomic regions specific to certain fungal species, has been associated with increased sensivity, allowing the detection of dormant forms of fungi, such as spores. We present several studies where both methods were used to detect the presence of toxigenic fungi, namely Aspergillus, particularly from the Fumigatus Flavi and Circumdati sections. |
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| Autores principais: | Gomes, Anita Quintal |
| Outros Autores: | Faria, Tiago; Caetano, Liliana Aranha; Sabino, Raquel; Viegas, Carla |
| Assunto: | Occupational health Occupational exposure Fungi IPL/2017/CalqPCR_ESTeSL |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Lisboa |
| Resumo: | Aim of the study - Fungal burden has traditionally been detected by conventional culture analysis, which despite its limitations, is widely used by the scientific community. Alternatively, quantitative real-time PCR (qPCR), based on the amplification of genomic regions specific to certain fungal species, has been associated with increased sensivity, allowing the detection of dormant forms of fungi, such as spores. We present several studies where both methods were used to detect the presence of toxigenic fungi, namely Aspergillus, particularly from the Fumigatus Flavi and Circumdati sections. |
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