Publicação
Bacterial contamination assessment and MRSA colonization in the context of occupational exposure in Portuguese swine productions
| Resumo: | Occupational health concern has been increasing worldwide in occupational settings with increased health-associated potential hazards such as direct contact with live animals. Animals, including pigs, are reservoirs of livestock-associated microorganisms, such as methicillin-resistant Staphylococcus aureus (MRSA), potentially linked with subsequent clinical diseases and transference of the infection/colonization to others. Here we aimed to perform a bacterial contamination characterization of the indoor environment and assess S. aureus colonization prevalence, including MRSA, in both workers and animals from five Portuguese swine productions. Nasopharyngeal samples (N=68) were collected and inoculated in non-selective and selective culture media and MRSA isolates were confirmed by immunologic assays. Air samples (50 L) were collected through an impaction method, seeded in TSA and VRBA media and quantitative colony-forming was obtained (CFU.m-3) after incubation. We report an exceptionally high prevalence of total bacteria and MRSA colonization in both workers and animals and demonstrated high air values of total bacterial concentration, potentially associated with a positive selection pressure. This work emphasizes the urge to monitor resistant bacteria strains in occupational contexts to develop proper surveillance programs to determine and regulate the antibiotic selection pressure that is driving the emergence of these resistant strains and the necessity to create occupational standards and take effective preventive measures. |
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| Autores principais: | Ribeiro, Edna |
| Outros Autores: | Monteiro, Ana |
| Assunto: | Environmental health Occupational health Bacterial contamination Antimicrobial resistance Swinneries IPL_2016-BBIORHealth Portugal |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico do Instituto Politécnico de Lisboa |
| Resumo: | Occupational health concern has been increasing worldwide in occupational settings with increased health-associated potential hazards such as direct contact with live animals. Animals, including pigs, are reservoirs of livestock-associated microorganisms, such as methicillin-resistant Staphylococcus aureus (MRSA), potentially linked with subsequent clinical diseases and transference of the infection/colonization to others. Here we aimed to perform a bacterial contamination characterization of the indoor environment and assess S. aureus colonization prevalence, including MRSA, in both workers and animals from five Portuguese swine productions. Nasopharyngeal samples (N=68) were collected and inoculated in non-selective and selective culture media and MRSA isolates were confirmed by immunologic assays. Air samples (50 L) were collected through an impaction method, seeded in TSA and VRBA media and quantitative colony-forming was obtained (CFU.m-3) after incubation. We report an exceptionally high prevalence of total bacteria and MRSA colonization in both workers and animals and demonstrated high air values of total bacterial concentration, potentially associated with a positive selection pressure. This work emphasizes the urge to monitor resistant bacteria strains in occupational contexts to develop proper surveillance programs to determine and regulate the antibiotic selection pressure that is driving the emergence of these resistant strains and the necessity to create occupational standards and take effective preventive measures. |
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