Publication
Construction of a stable manganese/copper core-shell laccase-like enzyme for recyclable dye decolorization and phenolic pollutant detection
| Summary: | Laccase shows significant potential for environmental remediation, yet its practical implementation is limited by inherent instability and restricted reusability. To address these limitations, we successfully synthesized a Mn/Cu-based core-shell structured laccase-like enzyme (SiO2@APTCu-MnO2) using a sol-gel method coupled with an in situ sacrificial strategy. Compared to natural laccase, SiO2@APTCu-MnO2 exhibited enhanced substrate affinity (Michaelis constant, Km = 0.29 mM) and a higher catalytic rate (Vmax = 1.05 ×10³ mM/min). It maintained exceptional stability across wide pH and temperature ranges, retaining over 80 % activity after 90 days. During application, the decolorization efficiency for anthraquinone and azo dyes was over 90 % within 2 h. Remarkably, it preserved over 80 % of its activity and decolorization capacity for reactive blue 19 even after 10 cycles. A dye decolorization-cultivation system confirmed that the treated water had minimal impact on plant growth. Additionally, the laccase-like enzyme enabled rapid and sensitive detection of phenolic pollutants. These results indicate that SiO2@APTCu-MnO serves as a high-performance, multifunctional alternative to natural laccase in environmental remediation and pollutant detection. |
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| Main Authors: | Chen, Xinyi |
| Other Authors: | Tao, Wenwen; Xu, Ruiguang; Mo, Taoling; Yang, Xue; Cavaco-Paulo, Artur; Su, Jing; Wang, Hongbo |
| Subject: | Core-shell structure Dye decolorization Laccase-like enzyme Phenolic pollutant detection |
| Year: | 2025 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| Summary: | Laccase shows significant potential for environmental remediation, yet its practical implementation is limited by inherent instability and restricted reusability. To address these limitations, we successfully synthesized a Mn/Cu-based core-shell structured laccase-like enzyme (SiO2@APTCu-MnO2) using a sol-gel method coupled with an in situ sacrificial strategy. Compared to natural laccase, SiO2@APTCu-MnO2 exhibited enhanced substrate affinity (Michaelis constant, Km = 0.29 mM) and a higher catalytic rate (Vmax = 1.05 ×10³ mM/min). It maintained exceptional stability across wide pH and temperature ranges, retaining over 80 % activity after 90 days. During application, the decolorization efficiency for anthraquinone and azo dyes was over 90 % within 2 h. Remarkably, it preserved over 80 % of its activity and decolorization capacity for reactive blue 19 even after 10 cycles. A dye decolorization-cultivation system confirmed that the treated water had minimal impact on plant growth. Additionally, the laccase-like enzyme enabled rapid and sensitive detection of phenolic pollutants. These results indicate that SiO2@APTCu-MnO serves as a high-performance, multifunctional alternative to natural laccase in environmental remediation and pollutant detection. |
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