Publicação
ΔG(CH2) in PEG-Salt and Ucon-Salt Aqueous Two-Phase Systems
| Resumo: | Three new phase diagrams for polymer−salt aqueous two-phase systems (ATPS) at 23 °C are presented. Then, partition coefficients for a series of five dinitrophenylated amino acids were obtained experimentally in five polymer−salt ATPS. The ATPS were obtained combining a polymer, polyethylene glycol (PEG) or Ucon (a random copolymer of 50 % ethylene oxide and 50 % propylene oxide), and a salt (Na2SO4, (NH4)2SO4, or Li2SO4). The series of amino acids allowed the calculation of the free energy of transfer of a methylene group, which is used as a measure of the relative hydrophobicity of the equilibrium phases. Ucon-rich phases proved to have a higher affinity for methylene groups than PEG which suggests a more efficient biphasic separation system with lower cross-contamination. |
|---|---|
| Autores principais: | Silvério, Sara C. |
| Outros Autores: | Madeira, Pedro; Rodríguez, Oscar; Teixeira, J. A.; Macedo, Eugénia |
| Ano: | 2008 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Three new phase diagrams for polymer−salt aqueous two-phase systems (ATPS) at 23 °C are presented. Then, partition coefficients for a series of five dinitrophenylated amino acids were obtained experimentally in five polymer−salt ATPS. The ATPS were obtained combining a polymer, polyethylene glycol (PEG) or Ucon (a random copolymer of 50 % ethylene oxide and 50 % propylene oxide), and a salt (Na2SO4, (NH4)2SO4, or Li2SO4). The series of amino acids allowed the calculation of the free energy of transfer of a methylene group, which is used as a measure of the relative hydrophobicity of the equilibrium phases. Ucon-rich phases proved to have a higher affinity for methylene groups than PEG which suggests a more efficient biphasic separation system with lower cross-contamination. |
|---|