Publicação
Study of biological activities of Cornus officinalis
| Resumo: | Traditional Chinese Medicine comprises a large number of medicinal plants used as source of many potent and powerful drugs. The search for drugs and the fight against idiseases led man to learn to use drug present in barks, roots, seeds, fruits and other parts of plants. Medicinal plants have been used for several millennia as pharmaceutical and dietetic therapy, because they are readily available and are cheaper than modern medicines. Their most common phytochemical compounds are alkaloids, glycosides, polyphenols and terpenes. They have the ability to interact with reactive species and play an important role in prevetion and treatment of various chronic diseases. Thus, knowledge the antioxidant content of plants can be harnessed enhancing the body’s antioxidant power. Antioxidants are the first line in oxidative stress defence agqainst the oxygen species (ROS) increase. They are molecules which act at low concentrations against the oxidation of a substract, preventing the accumulation of oxidized molecules and the extensive damage. The intake of natural antioxidants increase the body’s antioxidant power and promotes anti-inflammatory, antiatherosclerotic, anti-mutagenic, anticarcinogenic, antitumor and antibacterial activities. The growing interest in plants use as reinforcement of antioxidant defenses leads to the study of the protective activities of Cornus officinalis fruits. The first step of this investigation was the performance of a systematic review that portraying the bioactivities of this species as antiinflammatory, neuroprotective, antioxidant and antidibetic. The second step was the phytochemical and antioxidant characterization of a methanolic extract of C.officinalis fruits. The extract revealed to act by hydrogen atom transfer process reducing superoxide radicals, decreasing the formation of peroxil radicals through lipid peroxidantion and reducing iron ions. The third and last step, was evaluated the action of this extract in BV-2 and HepG2 cell lines to study their protective role, respectively in brain and liver. The extract allowed the cellular proliferation of HepG2 in normal conditions and protected these cells from oxidative damage. For the BV-2 cell line, the extract seemed to have some toxicity, being necessary the investigation of other parameters to advance possible protection studies. |
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| Autores principais: | Tavares, Ana Catarina Andrade |
| Assunto: | Cornus officinalis Oxidative Stress Reactie oxygen species BV-2 cell line HepG2 cell line Atividades biológicas Stresse oxidativo Espécis reativas de oxigénio Células BV-2 Células HepG2 |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Traditional Chinese Medicine comprises a large number of medicinal plants used as source of many potent and powerful drugs. The search for drugs and the fight against idiseases led man to learn to use drug present in barks, roots, seeds, fruits and other parts of plants. Medicinal plants have been used for several millennia as pharmaceutical and dietetic therapy, because they are readily available and are cheaper than modern medicines. Their most common phytochemical compounds are alkaloids, glycosides, polyphenols and terpenes. They have the ability to interact with reactive species and play an important role in prevetion and treatment of various chronic diseases. Thus, knowledge the antioxidant content of plants can be harnessed enhancing the body’s antioxidant power. Antioxidants are the first line in oxidative stress defence agqainst the oxygen species (ROS) increase. They are molecules which act at low concentrations against the oxidation of a substract, preventing the accumulation of oxidized molecules and the extensive damage. The intake of natural antioxidants increase the body’s antioxidant power and promotes anti-inflammatory, antiatherosclerotic, anti-mutagenic, anticarcinogenic, antitumor and antibacterial activities. The growing interest in plants use as reinforcement of antioxidant defenses leads to the study of the protective activities of Cornus officinalis fruits. The first step of this investigation was the performance of a systematic review that portraying the bioactivities of this species as antiinflammatory, neuroprotective, antioxidant and antidibetic. The second step was the phytochemical and antioxidant characterization of a methanolic extract of C.officinalis fruits. The extract revealed to act by hydrogen atom transfer process reducing superoxide radicals, decreasing the formation of peroxil radicals through lipid peroxidantion and reducing iron ions. The third and last step, was evaluated the action of this extract in BV-2 and HepG2 cell lines to study their protective role, respectively in brain and liver. The extract allowed the cellular proliferation of HepG2 in normal conditions and protected these cells from oxidative damage. For the BV-2 cell line, the extract seemed to have some toxicity, being necessary the investigation of other parameters to advance possible protection studies. |
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