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HPLC purification and structural characterization by NMR spectroscopy and mass spectrometry of Peptaibolin mimetics

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Detalhes bibliográficos
Resumo:This work presents our study of optimization of P-HPLC conditions to obtain pure peptaibol mimetics, to be used in biological activity studies. After purification, the sequence and structure of the peptides obtained were confirmed by NMR spectroscopy (1H, 13C, DEPT, HMBC and HMQC spectra) and mass spectrometry.
Autores principais:Castro, Vânia I. B.
Outros Autores:Carvalho, Carina Martins; Costa, Susana P. G.; Lima, Sílvia M. M. A. Pereira
Assunto:Peptide NMR spectroscopy Mass spectrometry HPLC purification
Ano:2014
País:Portugal
Tipo de documento:outro
Tipo de acesso:acesso restrito
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
Descrição
Resumo:This work presents our study of optimization of P-HPLC conditions to obtain pure peptaibol mimetics, to be used in biological activity studies. After purification, the sequence and structure of the peptides obtained were confirmed by NMR spectroscopy (1H, 13C, DEPT, HMBC and HMQC spectra) and mass spectrometry.