Publicação
Design of a 3D in vitro gastric model with in situ analysis for the assessment of food digestibility and nanomaterials behaviour
| Resumo: | The regular consumer is becoming more concern regarding their nutritional habits and, as such, novel healthier food products with additional functional properties must be developed to give response to the increase from the food market demand. However, while developing innovative food products through, for instance, the addition of nanosystems for the controlled delivery of bioactive compounds, it is important to understand their behaviour under digestion conditions by assessing their toxicity (i.e., the release of toxins during the digestion process), hydrolysis kinetics, nutrient absorption, etc. Ideally, in vivo trials should be used for this purpose since they represent as more accurately the GI conditions. However, due to all the ethical constrains, cost and complexity associated to their application, in vitro digestion models have been widely used as an alternative since they are faster, cheaper and less labour-intensive, with no ethical constrains. This way, several in vitro digestion models have been created to try to simulate the conditions of the human GI tract which have been widely used by the scientific community to study the digestion of food products, micro/nanosystems and drug dissolution. However, despite the development of a few anatomical accurate digestion models with dynamic behaviour over the past few years, there is still room for improvement. Therefore, the present study aims at the development of a modular dynamic in vitro RGM with in situ analysis, using UV-VIS-NIR spectrometry. This model is capable of replicating, as close as possible, the physicochemical conditions along the human stomach regarding its peristaltic contractions, as well as anatomical and physiological characteristics. The present model will allow to monitor in real-time the digestion process of food products, controlled release systems, bioactive compounds and drugs, thus being an important research, development and control tool for the food and pharmaceutical industries. |
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| Autores principais: | Madalena, Daniel Alexandre Silva |
| Assunto: | Food digestion In vitro digestion models Milk digestion Real-time analysis Machine learning Análise em tempo-real Digestão de alimentos Digestão de nanossistemas Modelos de digestão in vitro |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | tese de doutoramento |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | The regular consumer is becoming more concern regarding their nutritional habits and, as such, novel healthier food products with additional functional properties must be developed to give response to the increase from the food market demand. However, while developing innovative food products through, for instance, the addition of nanosystems for the controlled delivery of bioactive compounds, it is important to understand their behaviour under digestion conditions by assessing their toxicity (i.e., the release of toxins during the digestion process), hydrolysis kinetics, nutrient absorption, etc. Ideally, in vivo trials should be used for this purpose since they represent as more accurately the GI conditions. However, due to all the ethical constrains, cost and complexity associated to their application, in vitro digestion models have been widely used as an alternative since they are faster, cheaper and less labour-intensive, with no ethical constrains. This way, several in vitro digestion models have been created to try to simulate the conditions of the human GI tract which have been widely used by the scientific community to study the digestion of food products, micro/nanosystems and drug dissolution. However, despite the development of a few anatomical accurate digestion models with dynamic behaviour over the past few years, there is still room for improvement. Therefore, the present study aims at the development of a modular dynamic in vitro RGM with in situ analysis, using UV-VIS-NIR spectrometry. This model is capable of replicating, as close as possible, the physicochemical conditions along the human stomach regarding its peristaltic contractions, as well as anatomical and physiological characteristics. The present model will allow to monitor in real-time the digestion process of food products, controlled release systems, bioactive compounds and drugs, thus being an important research, development and control tool for the food and pharmaceutical industries. |
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