Publicação
Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery
| Resumo: | The influence of sonication power, suspension volume and cell concentration on the kinetics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of cytochrome b5. The sonication efficiency increased with the increase of sonication power and with the decrease of the suspension volume and cell concentration. |
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| Autores principais: | Santos, J. A. L. |
| Outros Autores: | Belo, Isabel; Mota, M.; Cabral, J. M. S. |
| Assunto: | Cell disruption Freeze/thawing Sonication Escherichia coli Cytochrome b5 |
| Ano: | 1996 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | The influence of sonication power, suspension volume and cell concentration on the kinetics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of cytochrome b5. The sonication efficiency increased with the increase of sonication power and with the decrease of the suspension volume and cell concentration. |
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