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A passive microfluidic device for cell sorting and deformability measurements

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Detalhes bibliográficos
Resumo:Passive microfluidic devices have long been a topic of interest due mainly to its associated low cost and easy fabrication, when compared with active microdevices, since passive microdevices do not need any electronic, mechanical or acoustic components. A passive microfluidic device with several stages of cross-flow filtration barriers and a sequence of three hyperbolic contractions in each outlet was used in this work. The experimental set-up has a high-speed camera combined with an inverted microscope where the image sequences were obtained at a frame rate of 2000 frames/s with a constant flow rate controlled by a syringe pump. The images sequences recorded during the flow visualizations were analyzed and processed by using image processing tools from the Image). software. It was measured the cells separation efficiency and it was obtained about 44% of RBCs separation from plasma. The deformability analysis has shown that the RBCs deformability is correlated with the presence of pathological blood.
Autores principais:Faustino, Vera
Outros Autores:Pinho, Diana; Catarino, Susana Oliveira; Miranda, João Mário; Minas, Graça; Lima, Rui Alberto Madeira Macedo
Assunto:Passive microfluidic devices RBCs sorting RBCs separation pathological blood
Ano:2018
País:Portugal
Tipo de documento:comunicação em conferência
Tipo de acesso:acesso restrito
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
Descrição
Resumo:Passive microfluidic devices have long been a topic of interest due mainly to its associated low cost and easy fabrication, when compared with active microdevices, since passive microdevices do not need any electronic, mechanical or acoustic components. A passive microfluidic device with several stages of cross-flow filtration barriers and a sequence of three hyperbolic contractions in each outlet was used in this work. The experimental set-up has a high-speed camera combined with an inverted microscope where the image sequences were obtained at a frame rate of 2000 frames/s with a constant flow rate controlled by a syringe pump. The images sequences recorded during the flow visualizations were analyzed and processed by using image processing tools from the Image). software. It was measured the cells separation efficiency and it was obtained about 44% of RBCs separation from plasma. The deformability analysis has shown that the RBCs deformability is correlated with the presence of pathological blood.