Publicação
Characterization of the malaria parasite optical features for development of a non-invasive diagnostic device
| Resumo: | [Excerpt] Early and accurate malaria diagnosis is critical for the disease control and elimination [1]. Microscopy and/or immuno-rapid tests remain the standard diagnosis [2, 3], nevertheless it requires a skin puncture for blood sampling and not sensitive enough for reliable detect lowdensity parasitemias, urging the need to develop more sensitive and non-invasive tools. Symptoms of the disease starts when parasites infect the red blood cell (RBC), suffering biochemical and morphological changes [4]. Parasite survival is dependent on hemozoin (Hz) formation as a by-product of heme detoxification process of the parasite upon haemoglobin (Hb) degradation and therefore a good unique feature to identify parasites presence in patients’. Taking advantage of the fact that the Hz and Hb molar extinction coefficients differ significantly, especially at certain wavelengths [4, 5], and their proportion is inversely related upon parasite maturation inside the RBC, each stage of malaria is characterized by specific absorbance and reflectance spectra, according to the Hb/Hz concentrations on the iRBC. [...] |
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| Autores principais: | Baptista, Vitória Cunha |
| Outros Autores: | Calçada, Carla Sofia Martins; Teixeira, Miguel Monteiro; Ferreira, Pedro Eduardo Mendes; Minas, Graça; Catarino, Susana Oliveira; Veiga, Maria Isabel |
| Assunto: | Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Engenharia Eletrotécnica, Eletrónica e Informática Engenharia e Tecnologia::Engenharia Médica |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | [Excerpt] Early and accurate malaria diagnosis is critical for the disease control and elimination [1]. Microscopy and/or immuno-rapid tests remain the standard diagnosis [2, 3], nevertheless it requires a skin puncture for blood sampling and not sensitive enough for reliable detect lowdensity parasitemias, urging the need to develop more sensitive and non-invasive tools. Symptoms of the disease starts when parasites infect the red blood cell (RBC), suffering biochemical and morphological changes [4]. Parasite survival is dependent on hemozoin (Hz) formation as a by-product of heme detoxification process of the parasite upon haemoglobin (Hb) degradation and therefore a good unique feature to identify parasites presence in patients’. Taking advantage of the fact that the Hz and Hb molar extinction coefficients differ significantly, especially at certain wavelengths [4, 5], and their proportion is inversely related upon parasite maturation inside the RBC, each stage of malaria is characterized by specific absorbance and reflectance spectra, according to the Hb/Hz concentrations on the iRBC. [...] |
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