Publicação
Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pretreated sugarcane straw
| Resumo: | Relatively few studies have addressed the characterization of sugarcane straw (SCS) for production fermentable sugars through enzyme hydrolysis. Straw is a major co-product of the sugarcane harvest in Brazil that has potential to sustainably increase cellulosic feedstocks in Brazil by 50%. Pretreatment of 10% w/v straw with liquid hot water (LHW) at 180°C for 50min (severity, So, of 4.05), solubilizes hemicellulose, preserves glucan, and generates 4.49g/L soluble phenolic compounds in the resulting liquid. Extracts from washing pretreated solids with hot excess water followed by acetone resulted in 1.10 and 0.83g/L phenolics, respectively. Acetone-derived extracts were more inhibitory and decreased glucose yield for enzyme hydrolysis of Solka Floc (a lignin-free cellulose) by 42%. In comparison, pretreated straw washed with hot water or acetone was readily hydrolyzed to 92% and 97% by cellulase enzyme. Hydrothermally treated SCS has the potential to provide a valuable and added source of fermentable sugars suitable for bioprocessing into biofuels and bioproducts when cellulase enzyme inhibitors are removed after pretreatment. |
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| Autores principais: | Michelin, Michele |
| Outros Autores: | Ximenes, Eduardo; Polizeli, Maria de Lourdes T. M.; Ladisch, Michael R. |
| Assunto: | Lignocellulose Enzyme inhibition Advanced biorefineries Phenolics Deactivation Inhibition Cellulase |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Relatively few studies have addressed the characterization of sugarcane straw (SCS) for production fermentable sugars through enzyme hydrolysis. Straw is a major co-product of the sugarcane harvest in Brazil that has potential to sustainably increase cellulosic feedstocks in Brazil by 50%. Pretreatment of 10% w/v straw with liquid hot water (LHW) at 180°C for 50min (severity, So, of 4.05), solubilizes hemicellulose, preserves glucan, and generates 4.49g/L soluble phenolic compounds in the resulting liquid. Extracts from washing pretreated solids with hot excess water followed by acetone resulted in 1.10 and 0.83g/L phenolics, respectively. Acetone-derived extracts were more inhibitory and decreased glucose yield for enzyme hydrolysis of Solka Floc (a lignin-free cellulose) by 42%. In comparison, pretreated straw washed with hot water or acetone was readily hydrolyzed to 92% and 97% by cellulase enzyme. Hydrothermally treated SCS has the potential to provide a valuable and added source of fermentable sugars suitable for bioprocessing into biofuels and bioproducts when cellulase enzyme inhibitors are removed after pretreatment. |
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