Publication
Orientational behaviors of silk fibroin hydrogels
| Summary: | In this study, a novel shear-induced silk fibroin hydrogel with three-dimensional (3D) anisotropic and oriented gel skeleton/network morphology is presented. Amphipathic anionic and nontoxic sodium surfactin is blended with the silk fibroin to decrease its gelation time during the mechanical shearing process. The fibroin/surfactin blended solutions undergo a facial shearing process to accomplish a solâ gel transition within one hour. The dynamic solâ gel transition kinetic analysis, gel skeleton/network morphology, and mechanical property measurements are determined in order to visualize the fibroin/surfactin solâ gel transition during the shearing process and its resulting hydrogel. The results demonstrate that there is significant b-sheet assembly from random coil conformations in the fibroin/surfactin blended system during the facile shearing process. The silk fibroin b-sheets further transform into a fibrous large-scale aggregation with orientational and parallel arrangements to the shearing direction. The shear-induced fibroin/ surfactin hydrogel exhibits notable anisotropic and oriented 3D skeleton/network morphology and a significant mechanical compressive strength in proportion to the shearing stress, compared with the control fibroin/surfactin hydrogel undergoing no shearing process. Due to its oriented gel skeleton/network structure and significantly enhanced mechanical properties, the shear-induced fibroin/ surfactin gel may be suitable as a biomaterial in 3D oriented tissue regeneration, including for nerves, the cultivation of bone cells, and the repair of defects in muscle and ligament tissues. |
|---|---|
| Main Authors: | Chen, Daqi |
| Other Authors: | Yin, Zhuping; Wu, Feng; Fu, Hua; Kundu, Subhas C; Lu, Shenzhou |
| Subject: | Biomaterials Gels Mechanical Properties Structure–property relationships |
| Year: | 2017 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| Summary: | In this study, a novel shear-induced silk fibroin hydrogel with three-dimensional (3D) anisotropic and oriented gel skeleton/network morphology is presented. Amphipathic anionic and nontoxic sodium surfactin is blended with the silk fibroin to decrease its gelation time during the mechanical shearing process. The fibroin/surfactin blended solutions undergo a facial shearing process to accomplish a solâ gel transition within one hour. The dynamic solâ gel transition kinetic analysis, gel skeleton/network morphology, and mechanical property measurements are determined in order to visualize the fibroin/surfactin solâ gel transition during the shearing process and its resulting hydrogel. The results demonstrate that there is significant b-sheet assembly from random coil conformations in the fibroin/surfactin blended system during the facile shearing process. The silk fibroin b-sheets further transform into a fibrous large-scale aggregation with orientational and parallel arrangements to the shearing direction. The shear-induced fibroin/ surfactin hydrogel exhibits notable anisotropic and oriented 3D skeleton/network morphology and a significant mechanical compressive strength in proportion to the shearing stress, compared with the control fibroin/surfactin hydrogel undergoing no shearing process. Due to its oriented gel skeleton/network structure and significantly enhanced mechanical properties, the shear-induced fibroin/ surfactin gel may be suitable as a biomaterial in 3D oriented tissue regeneration, including for nerves, the cultivation of bone cells, and the repair of defects in muscle and ligament tissues. |
|---|