Publicação

Heterologous production and purification of Candida albicans Cht3 and Sap2: novel recombinant subunit antigens

Detalhes bibliográficos
Resumo:	[Introdução] Candida albicans is an opportunistic human fungal pathogen responsible for a variety of infections in immunocompromised patients. Previous studies have shown that many cell wall proteins from C. albicans induce immunogenic responses against C. albicans infections. The secreted aspartyl proteinase 2 (Sap2) (1) is one of the best studied but a cell wall chitinase (Cht3) was also recently suggested to be involved. It was shown that a liposomal nanoparticle formulation containing encapsulated cell wall surface proteins (CWSP) had an immunoprotective potential against a lethal systemic candida infection in mice as a result of the production of antibodies specific to Cht3 (2). The overall aim of the present work is to determine the role of Cht3 in the immune response and assess its potential as a vaccination target against candidiasis. Such a study requires the availability of relatively large quantities of pure protein and hence we undertook a study to overexpress the recombinant protein and develop a suitable purification strategy. In addition, the protocol for production in Escherichia coli and purification of the C. albicans Sap2 was optimised for use as a control immunogen.
Autores principais:	Barbosa, Augusto
Outros Autores:	Sampaio, Paula; Collins, Tony
Ano:	2016
País:	Portugal
Tipo de documento:	póster em conferência
Tipo de acesso:	acesso restrito
Instituição associada:	Universidade do Minho
Idioma:	inglês
Origem:	RepositóriUM - Universidade do Minho

Descrição
Resumo:	[Introdução] Candida albicans is an opportunistic human fungal pathogen responsible for a variety of infections in immunocompromised patients. Previous studies have shown that many cell wall proteins from C. albicans induce immunogenic responses against C. albicans infections. The secreted aspartyl proteinase 2 (Sap2) (1) is one of the best studied but a cell wall chitinase (Cht3) was also recently suggested to be involved. It was shown that a liposomal nanoparticle formulation containing encapsulated cell wall surface proteins (CWSP) had an immunoprotective potential against a lethal systemic candida infection in mice as a result of the production of antibodies specific to Cht3 (2). The overall aim of the present work is to determine the role of Cht3 in the immune response and assess its potential as a vaccination target against candidiasis. Such a study requires the availability of relatively large quantities of pure protein and hence we undertook a study to overexpress the recombinant protein and develop a suitable purification strategy. In addition, the protocol for production in Escherichia coli and purification of the C. albicans Sap2 was optimised for use as a control immunogen.