Publicação
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
| Resumo: | Coagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis, are one of the leading causes of sepsis in preterm neonates mostly due to their capacity to form biofilms on medical devices. A major concern regarding CoNS infections is associated with the increased antibiotic resistance that has been observed among this bacterial group. Hence, the treatment of these infections is becoming progressively challenging, which may lead to increased morbidity and mortality in preterm neonates. In that sense, we aim to explore the consequences of antibiotic resistance in biofilm formation by S. epidermidis. First, the susceptibility profile of S. epidermidis strains collected from Portuguese newborns with bloodstream infections was characterized. To induce resistance to vancomycin, the susceptible strains were exposed to increasing concentrations of the antibiotic and, after 36 passages, the MIC was re-evaluated and the capacity of vancomycin-induced resistance strains to form biofilms was assessed (Colony forming units (CFUs) and OD620nm readings). Overall, the strains with induced resistance to vancomycin formed biofilms with less biomass and CFUs than the isogenic strains. The acquisition of resistance to vancomycin in clinical strains of S. epidermidis seems to cause a decrease in the capacity of these strains to form biofilms. As such, in S. epidermidis, the resistance to vancomycin does not seem to be related to a stronger biofilm formation capacity. |
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| Autores principais: | Santos, Inês Oliveira |
| Outros Autores: | França, Ângela Maria Oliveira Sousa |
| Assunto: | Staphylococcus epidermidis Biofilms Antibiotic resistance Vancomycin |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Coagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis, are one of the leading causes of sepsis in preterm neonates mostly due to their capacity to form biofilms on medical devices. A major concern regarding CoNS infections is associated with the increased antibiotic resistance that has been observed among this bacterial group. Hence, the treatment of these infections is becoming progressively challenging, which may lead to increased morbidity and mortality in preterm neonates. In that sense, we aim to explore the consequences of antibiotic resistance in biofilm formation by S. epidermidis. First, the susceptibility profile of S. epidermidis strains collected from Portuguese newborns with bloodstream infections was characterized. To induce resistance to vancomycin, the susceptible strains were exposed to increasing concentrations of the antibiotic and, after 36 passages, the MIC was re-evaluated and the capacity of vancomycin-induced resistance strains to form biofilms was assessed (Colony forming units (CFUs) and OD620nm readings). Overall, the strains with induced resistance to vancomycin formed biofilms with less biomass and CFUs than the isogenic strains. The acquisition of resistance to vancomycin in clinical strains of S. epidermidis seems to cause a decrease in the capacity of these strains to form biofilms. As such, in S. epidermidis, the resistance to vancomycin does not seem to be related to a stronger biofilm formation capacity. |
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