Publicação
Advances in the diversity of Acinetobacter phage-encoded capsular depolymerases
| Resumo: | [Excerpt] Introduction: Carbapenem-resistant Acinetobacter baumannii (CRAB) are bacteria resistant to nearly all antibiotics and difficult to remove from the environment. This bacterium displays diverse capsule types (K-types) to protect themselves from environmental pressures, including viral predation. In turn, bacteriophages have evolved to encode capsular depolymerases to recognize specific K-types, making their host range very narrow. Objective: To expand the collection of capsular depolymerases for different A. baumannii capsule types. Additionally, to study the phage depolymerase-host interactions, using recombinantly expressed depolymerases or engineered phages, having heterologous capsular enzymatic activities. Methodology: In silico, we analyzed all Acinetobacter phage genomes to search for putative capsular depolymerases. In vitro, depolymerases from several phages were cloned and validated using drop tests. Finally, some depolymerases were also introduced into A. baumannii phages by homologous recombination. The genes were introduced to replace native capsular depolymerase sequences. [...] |
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| Autores principais: | Oliveira, Hugo Alexandre Mendes |
| Outros Autores: | Domingues, Rita; Vieira, Maria; Gomes, Marta; Dias, Oscar; Azeredo, Joana |
| Assunto: | Acinetobacter Bacteriophage Therapy Artificial Intelligence |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | português |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | [Excerpt] Introduction: Carbapenem-resistant Acinetobacter baumannii (CRAB) are bacteria resistant to nearly all antibiotics and difficult to remove from the environment. This bacterium displays diverse capsule types (K-types) to protect themselves from environmental pressures, including viral predation. In turn, bacteriophages have evolved to encode capsular depolymerases to recognize specific K-types, making their host range very narrow. Objective: To expand the collection of capsular depolymerases for different A. baumannii capsule types. Additionally, to study the phage depolymerase-host interactions, using recombinantly expressed depolymerases or engineered phages, having heterologous capsular enzymatic activities. Methodology: In silico, we analyzed all Acinetobacter phage genomes to search for putative capsular depolymerases. In vitro, depolymerases from several phages were cloned and validated using drop tests. Finally, some depolymerases were also introduced into A. baumannii phages by homologous recombination. The genes were introduced to replace native capsular depolymerase sequences. [...] |
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