Publicação

A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)

Ver documento

Detalhes bibliográficos
Resumo:Separation of a large number of viable nematodes is essential for physiological srudies on Sleinernema carpocapsae. In this paper, we describe a fast and useful method for the separation of adults of this nematode species. This method relies on the determination of the densiry of males and females by applying a modified version of Stokes' law. Adults were separated in a discontinuous sucrase gradient at 10 and 20 %. Males and females separated by this method were 95 % viable.
Autores principais:Neves, J. M.
Outros Autores:Mota, M.; Simões, N.; Teixeira, J. A.
Assunto:Gradient Separation Steinernema carpocapsae Sucrose
Ano:1996
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
Descrição
Resumo:Separation of a large number of viable nematodes is essential for physiological srudies on Sleinernema carpocapsae. In this paper, we describe a fast and useful method for the separation of adults of this nematode species. This method relies on the determination of the densiry of males and females by applying a modified version of Stokes' law. Adults were separated in a discontinuous sucrase gradient at 10 and 20 %. Males and females separated by this method were 95 % viable.