Publicação

Indigo backstaining during cellulase washing

Detalhes bibliográficos
Resumo:	We have attempted to understand the mechanisms of indigo backstaining during enzyme stone washing by separately analyzing dye staining levels on different cotton surfaces during the process. The high ability of the cellulase enzyme protein to bind to cotton cellulose is the major cause of backstaining. Further studies of commercial cellulases show that the pH of maximum cellulase activity may not be the same pH of maximum of cellulase binding capacity to the substrate, and also that indigo dyes have completely different affinities for cellulase proteins from different fungal origins.
Autores principais:	Paulo, Artur Cavaco
Outros Autores:	Morgado, José; Almeida, Luís de; Kilburn, Douglas
Ano:	1998
País:	Portugal
Tipo de documento:	artigo
Tipo de acesso:	acesso aberto
Instituição associada:	Universidade do Minho
Idioma:	inglês
Origem:	RepositóriUM - Universidade do Minho

Descrição
Resumo:	We have attempted to understand the mechanisms of indigo backstaining during enzyme stone washing by separately analyzing dye staining levels on different cotton surfaces during the process. The high ability of the cellulase enzyme protein to bind to cotton cellulose is the major cause of backstaining. Further studies of commercial cellulases show that the pH of maximum cellulase activity may not be the same pH of maximum of cellulase binding capacity to the substrate, and also that indigo dyes have completely different affinities for cellulase proteins from different fungal origins.