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Indigo backstaining during cellulase washing

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Resumo:We have attempted to understand the mechanisms of indigo backstaining during enzyme stone washing by separately analyzing dye staining levels on different cotton surfaces during the process. The high ability of the cellulase enzyme protein to bind to cotton cellulose is the major cause of backstaining. Further studies of commercial cellulases show that the pH of maximum cellulase activity may not be the same pH of maximum of cellulase binding capacity to the substrate, and also that indigo dyes have completely different affinities for cellulase proteins from different fungal origins.
Autores principais:Paulo, Artur Cavaco
Outros Autores:Morgado, José; Almeida, Luís de; Kilburn, Douglas
Ano:1998
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Paulo, Artur Cavaco
author2 Morgado, José
Almeida, Luís de
Kilburn, Douglas
author2_role author
author
author
author_facet Paulo, Artur Cavaco
Morgado, José
Almeida, Luís de
Kilburn, Douglas
author_role author
contributor_name_str_mv RepositóriUM - Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Paulo, Artur Cavaco\"},{\"Person.name\":\"Morgado, José\"},{\"Person.name\":\"Almeida, Luís de\"},{\"Person.name\":\"Kilburn, Douglas\"}]
datacite.contributors.contributor.contributorName.fl_str_mv RepositóriUM - Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Paulo, Artur Cavaco
Morgado, José
Almeida, Luís de
Kilburn, Douglas
datacite.date.Accepted.fl_str_mv 1998-06-01T00:00:00Z
datacite.date.available.fl_str_mv 2006-09-28T13:35:11Z
datacite.date.embargoed.fl_str_mv 2006-09-28T13:35:11Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.titles.title.fl_str_mv Indigo backstaining during cellulase washing
dc.contributor.none.fl_str_mv RepositóriUM - Universidade do Minho
dc.creator.none.fl_str_mv Paulo, Artur Cavaco
Morgado, José
Almeida, Luís de
Kilburn, Douglas
dc.date.Accepted.fl_str_mv 1998-06-01T00:00:00Z
dc.date.available.fl_str_mv 2006-09-28T13:35:11Z
dc.date.embargoed.fl_str_mv 2006-09-28T13:35:11Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/5616
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv SAGE Publications
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.title.fl_str_mv Indigo backstaining during cellulase washing
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description We have attempted to understand the mechanisms of indigo backstaining during enzyme stone washing by separately analyzing dye staining levels on different cotton surfaces during the process. The high ability of the cellulase enzyme protein to bind to cotton cellulose is the major cause of backstaining. Further studies of commercial cellulases show that the pH of maximum cellulase activity may not be the same pH of maximum of cellulase binding capacity to the substrate, and also that indigo dyes have completely different affinities for cellulase proteins from different fungal origins.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://repositorium.uminho.pt/bitstreams/6567825d-b72e-4539-bb06-2663b2146a72/download
id rum_79b760113b7c1947dd1079f2e3a2bd77
identifier.url.fl_str_mv https://hdl.handle.net/1822/5616
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
network_acronym_str rum
network_name_str RepositóriUM - Universidade do Minho
oai_identifier_str oai:repositorium.uminho.pt:1822/5616
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Paulo, Artur Cavaco
Morgado, José
Almeida, Luís de
Kilburn, Douglas
publishDate 1998
publisher.none.fl_str_mv SAGE Publications
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engSAGE PublicationsporWe have attempted to understand the mechanisms of indigo backstaining during enzyme stone washing by separately analyzing dye staining levels on different cotton surfaces during the process. The high ability of the cellulase enzyme protein to bind to cotton cellulose is the major cause of backstaining. Further studies of commercial cellulases show that the pH of maximum cellulase activity may not be the same pH of maximum of cellulase binding capacity to the substrate, and also that indigo dyes have completely different affinities for cellulase proteins from different fungal origins.application/pdfengIndigo backstaining during cellulase washingPaulo, Artur CavacoMorgado, JoséAlmeida, Luís deKilburn, DouglasHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATION"Textile Research Journal". ISSN 0040-5175. 68:6 (1998) 398-401.ISSNIsPartOf0040-5175DOIIsPartOf10.1177/0040517598068006022006-09-28T13:35:11Z1998-061998-06-01T00:00:00ZHandlehttps://hdl.handle.net/1822/5616http://purl.org/coar/access_right/c_abf2open access1860540 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/6567825d-b72e-4539-bb06-2663b2146a72/download
spellingShingle Indigo backstaining during cellulase washing
Paulo, Artur Cavaco
status SINGLETON
title Indigo backstaining during cellulase washing
title_full Indigo backstaining during cellulase washing
title_fullStr Indigo backstaining during cellulase washing
title_full_unstemmed Indigo backstaining during cellulase washing
title_short Indigo backstaining during cellulase washing
title_sort Indigo backstaining during cellulase washing
url https://hdl.handle.net/1822/5616
visible 1