Publicação

Exploring syntrophic relationships in the anaerobic biodegradation of lipids and long chain fatty acids

Detalhes bibliográficos
Resumo:	[Excerpt] Practical knowledge on anaerobic digestion of waste lipids has been improving for several decades, but the microbiology of these processes remains partially undisclosed, with non-cultivated taxonomic groups often detected in anaerobic communities degrading lipids. This work studies the diversity and physiology of anaerobic microorganisms involved in the metabolism of lipids and long chain fatty acids. Anaerobic culturing procedures were applied for the development of enrichment cultures, and combined with next generation sequencing techniques. Enriched microbial communities specialized in the degradation of triolein (0.3 mmol·L-1) and oleate (1 mmol·L-1) were obtained under methanogenic conditions. Oleatedegrading cultures were also developed in the presence of the external electron acceptors ferric hydroxide (75 mmol·L-1) or sulfate (15 mmol·L-1). Three mesophilic sludges from different origins were used as inocula. [...]
Autores principais:	Cavaleiro, Ana Júlia
Outros Autores:	Ribeiro, J.; Guedes, Ana P.; Pereira, Ana Luísa Arantes Carvalho; Pereira, M. A.; Sousa, Diana Zita Machado; Alves, M. M.; Stams, Alfons Johannes Maria
Ano:	2017
País:	Portugal
Tipo de documento:	outro
Tipo de acesso:	acesso aberto
Instituição associada:	Universidade do Minho
Idioma:	inglês
Origem:	RepositóriUM - Universidade do Minho

Descrição
Resumo:	[Excerpt] Practical knowledge on anaerobic digestion of waste lipids has been improving for several decades, but the microbiology of these processes remains partially undisclosed, with non-cultivated taxonomic groups often detected in anaerobic communities degrading lipids. This work studies the diversity and physiology of anaerobic microorganisms involved in the metabolism of lipids and long chain fatty acids. Anaerobic culturing procedures were applied for the development of enrichment cultures, and combined with next generation sequencing techniques. Enriched microbial communities specialized in the degradation of triolein (0.3 mmol·L-1) and oleate (1 mmol·L-1) were obtained under methanogenic conditions. Oleatedegrading cultures were also developed in the presence of the external electron acceptors ferric hydroxide (75 mmol·L-1) or sulfate (15 mmol·L-1). Three mesophilic sludges from different origins were used as inocula. [...]