Publicação
Bioinspired three-dimensional magnetoactive scaffolds for bone tissue engineering
| Resumo: | Bone tissue repair strategies are gaining increasing relevance due to the growing incidence of bone disorders worldwide. Biochemical stimulation is the most commonly used strategy for cell regeneration while the application of physical stimuli such as magnetic, mechanical or electrical fields is a promising, however scarcely investigated field. This work reports on novel magneto-active 3D porous scaffolds, suitable for effective proliferation of osteoblasts in a biomimetic microenvironment. This physically active microenvironment is developed through the bone mimicking structure of the scaffold combined with the physical stimuli provided by a magnetic custom-made bioreactor on a magneto-responsive scaffold. Scaffolds are obtained through the development of nanocomposites comprised of a piezoelectric polymer, poly(vinylidene fluoride) (PVDF), and magnetostrictive particles of CoFe2O4 using a solvent casting method guided by the overlapping of nylon template structures with three different fibre diameter sizes (60, 80 and 120 µm), thus generating 3D scaffolds with different pore sizes. The magneto-active composites show a structure very similar to trabecular bone with pore sizes ranging from 5 µm to 20 µm, owed to the inherent process of crystallization of PVDF with the NPs, interconnected with bigger pores, formed after removing the nylon templates. It is found that the materials crystallize mainly in the electroactive -phase of PVDF and that promote the proliferation of pre-osteoblasts through the application of magnetic stimuli. This phenomenon is attributed to both local magnetomechanical and magnetoelectric response of the scaffolds, which induce a proper cellular mechano- and electro-transduction process. |
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| Autores principais: | Fernandes, Margarida Maria Macedo |
| Outros Autores: | Correia, Daniela Maria Silva; Ribeiro, Clarisse; Castro, Nelson; Correia, Vitor; Lanceros-Méndez, S. |
| Assunto: | 3D scaffolds magnetic stimuli magnetomechanical effect magnetoelectrical effect biomimetic bone tissue engineering |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Bone tissue repair strategies are gaining increasing relevance due to the growing incidence of bone disorders worldwide. Biochemical stimulation is the most commonly used strategy for cell regeneration while the application of physical stimuli such as magnetic, mechanical or electrical fields is a promising, however scarcely investigated field. This work reports on novel magneto-active 3D porous scaffolds, suitable for effective proliferation of osteoblasts in a biomimetic microenvironment. This physically active microenvironment is developed through the bone mimicking structure of the scaffold combined with the physical stimuli provided by a magnetic custom-made bioreactor on a magneto-responsive scaffold. Scaffolds are obtained through the development of nanocomposites comprised of a piezoelectric polymer, poly(vinylidene fluoride) (PVDF), and magnetostrictive particles of CoFe2O4 using a solvent casting method guided by the overlapping of nylon template structures with three different fibre diameter sizes (60, 80 and 120 µm), thus generating 3D scaffolds with different pore sizes. The magneto-active composites show a structure very similar to trabecular bone with pore sizes ranging from 5 µm to 20 µm, owed to the inherent process of crystallization of PVDF with the NPs, interconnected with bigger pores, formed after removing the nylon templates. It is found that the materials crystallize mainly in the electroactive -phase of PVDF and that promote the proliferation of pre-osteoblasts through the application of magnetic stimuli. This phenomenon is attributed to both local magnetomechanical and magnetoelectric response of the scaffolds, which induce a proper cellular mechano- and electro-transduction process. |
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