Publicação
Development of high-sensitivity colorimetric assay for detection of multiple urinary tract infection biomarkers on diaper
| Resumo: | The invasion and the multiplication of microorganisms like bacteria, viruses and parasites that are not commonly found on the human body may cause infections. Regarding incidence it is important to point out the urinary tract infections (UTI), the second most common kind of infection, making of utmost importance its early detection, diagnosis and swift identification of the most appropriate customized treatment. These infections can be detected using urinalysis that can also handle the diagnosis of conditions such as kidney and liver disorders and diabetes. The diagnostic methods currently used for detection of UTI are the dipsticks, microscopic examination, urine culture and automated urinalysis that require urine collection and analysis, often a painful and time-consuming procedure. During the last two decades, novel approaches based on miniaturized devices have been conceived. In this dissertation, a paper-based microfluidic device fabricated with inexpensive materials for urinalysis was designed, fabricated and tested. This device is attached on a diaper and aims to collect urine samples and determine quantitatively the amount of specific urine components such as blood, leukocyte esterase, glucose, proteins and nitrite. This new design is proposed to overcome the unmet limitations of existing technologies such as the isolation of the analytical device from the external environment and the communication between reagent pads. The solution for the first problem was attempted through the implementation of a swelling-based “self-locking” mechanism; as for the second issue, hydrophobic channels on filter paper and a layer with frames were created. The performance of device was tested by placing it in a surface, mimetizing its application in the diaper and water was dropped on top of it. It was possible to demonstrate that the “self-locking” mechanism was effective; on the other hand, interferences arise between reagent pads, probably due to capillary flow between the layers. In order to optimize this design a second generation of the device has been conceived and is presented. Overall, the approach used seems promising, since a single devices is able to collect, detect and analyze a urine sample in a short period, making the patients’ life more comfortable, being in addition portable and easy to operate. |
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| Autores principais: | Couto, Adriana Miguel Serra Silva |
| Assunto: | Urinalysis Microfluidics Paper-based devices Hydrogel Análise de urina Microfluídica Dispositivos em papel |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | The invasion and the multiplication of microorganisms like bacteria, viruses and parasites that are not commonly found on the human body may cause infections. Regarding incidence it is important to point out the urinary tract infections (UTI), the second most common kind of infection, making of utmost importance its early detection, diagnosis and swift identification of the most appropriate customized treatment. These infections can be detected using urinalysis that can also handle the diagnosis of conditions such as kidney and liver disorders and diabetes. The diagnostic methods currently used for detection of UTI are the dipsticks, microscopic examination, urine culture and automated urinalysis that require urine collection and analysis, often a painful and time-consuming procedure. During the last two decades, novel approaches based on miniaturized devices have been conceived. In this dissertation, a paper-based microfluidic device fabricated with inexpensive materials for urinalysis was designed, fabricated and tested. This device is attached on a diaper and aims to collect urine samples and determine quantitatively the amount of specific urine components such as blood, leukocyte esterase, glucose, proteins and nitrite. This new design is proposed to overcome the unmet limitations of existing technologies such as the isolation of the analytical device from the external environment and the communication between reagent pads. The solution for the first problem was attempted through the implementation of a swelling-based “self-locking” mechanism; as for the second issue, hydrophobic channels on filter paper and a layer with frames were created. The performance of device was tested by placing it in a surface, mimetizing its application in the diaper and water was dropped on top of it. It was possible to demonstrate that the “self-locking” mechanism was effective; on the other hand, interferences arise between reagent pads, probably due to capillary flow between the layers. In order to optimize this design a second generation of the device has been conceived and is presented. Overall, the approach used seems promising, since a single devices is able to collect, detect and analyze a urine sample in a short period, making the patients’ life more comfortable, being in addition portable and easy to operate. |
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