Detalhes bibliográficos
| Resumo: | A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 03.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.65.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.910 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1100 µg/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 ± 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release. |
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| Autores principais: | Rigon, Roberta B. |
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| Outros Autores: | Fachinetti, Naiara; Severino, Patrícia; Durazzo, Alessandra; Lucarini, Massimo; Atanasov, Atanas G.; El Mamouni, Soukaina; Chorilli, Marlus; Santini, Antonello; Souto, Eliana B. |
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| Assunto: | trans-resveratrol nanotechnology solid lipid nanoparticles high-performance liquid chromatography |
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| Ano: | 2019 |
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| País: | Portugal |
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| Tipo de documento: | artigo |
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| Tipo de acesso: | acesso aberto |
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| Instituição associada: | Universidade do Minho |
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| Idioma: | inglês |
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| Origem: | RepositóriUM - Universidade do Minho |
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