Publicação
Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches
| Resumo: | Aptamers are structural single-stranded oligonucleotides generated in vitro to bind to a specific target molecule. Aptamers versatility can be enhanced with nucleic acid mimics (NAMs) during or after a selection process, also known as systematic evolution of ligands by exponential enrichment (SELEX). We address advantages and limitations of the technologies used to generate NAM aptamers, especially the applicability of existing engineered polymerases to replicate NAMs and methodologies to improve aptamers after SELEX. We also discuss the limitations of existing methods for sequencing NAM sequences and bioinformatic tools to predict NAM aptamer structures. As a conclusion, we suggest that NAM aptamers might successfully compete with molecular tools based on proteins such as antibodies for future application. |
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| Autores principais: | Oliveira, Ricardo |
| Outros Autores: | Pinho, Eva; Sousa, Ana Luísa; DeStefano, Jeffrey J.; Azevedo, Nuno Filipe; Almeida, Carina |
| Assunto: | aptamers systematic evolution of ligands by exponential enrichment (SELEX) post-SELEX modifications nucleic acid mimics engineered polymerases xeno nucleic acids de novo systematic evolution of ligands by exponential enrichment (SELEX) |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Aptamers are structural single-stranded oligonucleotides generated in vitro to bind to a specific target molecule. Aptamers versatility can be enhanced with nucleic acid mimics (NAMs) during or after a selection process, also known as systematic evolution of ligands by exponential enrichment (SELEX). We address advantages and limitations of the technologies used to generate NAM aptamers, especially the applicability of existing engineered polymerases to replicate NAMs and methodologies to improve aptamers after SELEX. We also discuss the limitations of existing methods for sequencing NAM sequences and bioinformatic tools to predict NAM aptamer structures. As a conclusion, we suggest that NAM aptamers might successfully compete with molecular tools based on proteins such as antibodies for future application. |
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